ArticleThe Journal of experimental medicine1993
The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes.
Article in The Journal of experimental medicine, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 137 citations in OpenAlex.
- Article
- The Involvement of Alarmins in the Pathogenesis of Sjögren's Syndrome.International journal of molecular sciences · 2022Review
- Cancer Immune Therapy for Philadelphia Chromosome-Negative Chronic Myeloproliferative Neoplasms.Cancers · 2020Review
- Knocking 'em Dead: Pore-Forming Proteins in Immune Defense.Annual review of immunology · 2020Review
- Immunogenicity of cell death driven by immune effectors.Journal for immunotherapy of cancer · 2020Article
- To Kill But Not Be Killed: Controlling the Activity of Mammalian Pore-Forming Proteins.Frontiers in immunology · 2020Review
- CD8Frontiers in immunology · 2019Review
- Novel molecular mechanism of cellular transformation by a mutant molecular chaperone in myeloproliferative neoplasms.Cancer science · 2017Review
- High-level secretion of native recombinant human calreticulin in yeast.Microbial cell factories · 2015Article
- The role of heat shock proteins in atherosclerosis.Nature reviews. Cardiology · 2014Review
- Calreticulin and cancer.Pathology oncology research : POR · 2013Review
- Calreticulin in the immune system: ins and outs.Trends in immunology · 2013Review
- Proteasomal degradation of the metabotropic glutamate receptor 1α is mediated by Homer-3 via the proteasomal S8 ATPase: Signal transduction and synaptic transmission.Journal of neurochemistry · 2012Article
- Exocytosis and protein secretion in Trypanosoma.BMC microbiology · 2010Article
- Excreted/secreted proteins from trypanosome procyclic strains.Journal of biomedicine & biotechnology · 2010Article
- Proteomic identification of multitasking proteins in unexpected locations complicates drug targeting.Nature reviews. Drug discovery · 2009Review
- Delivering the kiss of death: progress on understanding how perforin works.Current opinion in immunology · 2007Review
- Calreticulin binds preferentially with B cell linear epitopes of Ro60 kD autoantigen, enhancing recognition by anti-Ro60 kD autoantibodies.Clinical and experimental immunology · 2003Article
- The ABCs of granule-mediated cytotoxicity: new weapons in the arsenal.Nature reviews. Immunology · 2003Review
- Endoplasmic reticulum in the heart, a forgotten organelle?Molecular and cellular biochemistry · 2001Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytolytic T lymphocytes (CTL), natural killer cells, and lymphokine-activated killer (LAK) cells are cytolytic cells known to release the cytolytic protein perforin and a family of proteases, named granzymes, from cytoplasmic stores upon interaction with target cells. We now report the purification of an additional major 60-kD granule-associated protein (grp 60) from human LAK cells and from mouse cytolytic T cells. The NH2-terminal amino acid sequence of the polypeptide was found to be identical to calreticulin. Calreticulin is a calcium storage protein and carries a COOH-terminal KDEL sequence, known to act as a retention signal for proteins destined to the lumen of the endoplasmic reticulum. In CTLs, however, calreticulin colocalizes with the lytic perforin to the lysosome-like secretory granules, as confirmed by double label immunofluorescence confocal microscopy. Moreover, when the release of granule-associated proteins was triggered by stimulation of the T cell receptor complex, calreticulin was released along with granzymes A and D. Since perforin is activated and becomes lytic in the presence of calcium, we propose that the role of calreticulin is to prevent organelle autolysis due to the protein's calcium chelator capacity.
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