ArticleMolecular and cellular biology1993
Involvement of rho p21 and its inhibitory GDP/GTP exchange protein (rho GDI) in cell motility.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 197 citations in OpenAlex.
- Article
- RhoGDI2 expression in astrocytes after an excitotoxic lesion in the mouse hippocampus.Cellular and molecular neurobiology · 2015Article
- The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility.Experimental cell research · 2014Article
- p104 binds to Rac1 and reduces its activity during myotube differentiation of C2C12 cell.TheScientificWorldJournal · 2014Article
- RhoGDIα downregulates androgen receptor signaling in prostate cancer cells.The Prostate · 2013Article
- DEK depletion negatively regulates Rho/ROCK/MLC pathway in non-small cell lung cancer.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- Increased cytoplasmic localization of p27(kip1) and its modulation of RhoA activity during progression of chronic myeloid leukemia.PloS one · 2013Article
- RhoGDIα suppresses growth and survival of prostate cancer cells.The Prostate · 2012Article
- A novel role of Rho-kinase in the regulation of ligand-induced phosphorylated EGFR endocytosis via the early/late endocytic pathway in human fibrosarcoma cells.Journal of molecular histology · 2011Article
- Rho GDP dissociation inhibitor 2 suppresses metastasis via unconventional regulation of RhoGTPases.Cancer research · 2009Article
- Therapeutic IMC-C225 antibody inhibits breast cancer cell invasiveness via Vav2-dependent activation of RhoA GTPase.Clinical cancer research : an official journal of the American Association for Cancer Research · 2008Article
- Kank regulates RhoA-dependent formation of actin stress fibers and cell migration via 14-3-3 in PI3K-Akt signaling.The Journal of cell biology · 2008Article
- RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells.Experimental cell research · 2008Article
- Localization and translocation of RhoA protein in the human gastric cancer cell line SGC-7901.World journal of gastroenterology · 2008Article
- GDI-1 phosphorylation switch at serine 96 induces RhoA activation and increased endothelial permeability.Molecular and cellular biology · 2007Article
- The small GTPase Rac is involved in clustering of hippocampal neurons and fasciculation of their neurites.Naunyn-Schmiedeberg's archives of pharmacology · 2004Article
- FAK induces expression of Prx1 to promote tenascin-C-dependent fibroblast migration.The Journal of cell biology · 2003Article
- Overexpression of ROCK in human breast cancer cells: evidence that ROCK activity mediates intracellular membrane traffic of lysosomes.Pathology oncology research : POR · 2003Article
- A role for small GTPase RhoA in regulating intracellular membrane traffic of lysosomes in invasive rat hepatoma cells.The Histochemical journal · 2002Article
- Lovastatin alters cytoskeleton organization and inhibits experimental metastasis of mammary carcinoma cells.Clinical & experimental metastasis · 2002Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Evidence is accumulating that rho p21, a ras p21-related small GTP-binding protein (G protein), regulates the actomyosin system. The actomyosin system is known to be essential for cell motility. In the present study, we examined the action of rho p21, its inhibitory GDP/GTP exchange protein (named rho GDI), its stimulatory GDP/GTP exchange protein (named smg GDS), and Clostridium botulinum ADP-ribosyltransferase C3, known to selectively ADP-ribosylate rho p21 and to impair its function, in cell motility (chemokinesis) of Swiss 3T3 cells. We quantitated the capacity of cell motility by measuring cell tracks by phagokinesis. Microinjection of the GTP gamma S-bound active form of rhoA p21 or smg GDS into Swiss 3T3 cells did not affect cell motility, but microinjection of rho GDI into the cells did inhibit cell motility. This rho GDI action was prevented by comicroinjection of rho GDI with the GTP gamma S-bound form of rhoA p21 but not with the same form of rhoA p21 lacking the C-terminal three amino acids which was not posttranslationally modified with lipids. The rho GDI action was not prevented by Ki-rasVal-12 p21 or any of the GTP gamma S-bound form of other small GTP-binding proteins including rac1 p21, G25K, and smg p21B. Among these small G proteins, rhoA p21, rac1 p21, and G25K are known to be substrates for rho GDI. The rho GDI action was not prevented by comicroinjection of rho GDI with smg GDS. Microinjection of C3 into Swiss 3T3 cells also inhibited cell motility. These results indicate that the rho GDI-rho p21 system regulates cell motility, presumably through the actomyosin system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.