ArticleMolecular and cellular biology1993
Ras activation by insulin and epidermal growth factor through enhanced exchange of guanine nucleotides on p21ras.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 174 citations in OpenAlex.
- Cell cycle control by the insulin-like growth factor signal: at the crossroad between cell growth and mitotic regulation.Cell cycle (Georgetown, Tex.) · 2023Review
- Pleiotropic Roles of Atrial Natriuretic Peptide in Anti-Inflammation and Anti-Cancer Activity.Cancers · 2022Review
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- Impact of Lifestyle Behaviors on Cancer Risk and Prevention.Journal of lifestyle medicine · 2021Review
- Underrated enemy - from nonalcoholic fatty liver disease to cancers of the gastrointestinal tract.Clinical and experimental hepatology · 2018Review
- Feedback activation of neurofibromin terminates growth factor-induced Ras activation.Cell communication and signaling : CCS · 2016Article
- Starvation based differential chemotherapy: a novel approach for cancer treatment.Oman medical journal · 2014Review
- Mitogen-Activated Protein (MAP) Kinase Scaffolding Proteins: A Recount.International journal of molecular sciences · 2013Article
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- Fasting vs dietary restriction in cellular protection and cancer treatment: from model organisms to patients.Oncogene · 2011Review
- Mechanism of the mitogenic influence of hyperinsulinemia.Diabetology & metabolic syndrome · 2011Article
- Article
- Mitogenic action of insulin: friend, foe or 'frenemy'?Diabetologia · 2010Article
- Systems-level interactions between insulin-EGF networks amplify mitogenic signaling.Molecular systems biology · 2009Article
- A Ras-induced conformational switch in the Ras activator Son of sevenless.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Ras activation in response to lysophosphatidic acid requires a permissive input from the epidermal growth factor receptor.The Biochemical journal · 2003Article
- A unified model for signal transduction reactions in cellular membranes.Biophysical journal · 2002Article
- Differential requirements for ERK1/2 and P38 MAPK activation by thrombin in T cells. Role of P59Fyn and PKCepsilon.Oncogene · 2001Article
- Review
- Functional Rac-1 and Nck signaling networks are required for FGF-2-induced DNA synthesis in MCF-7 cells.Oncogene · 1999Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A number of growth factors, including insulin and epidermal growth factor (EGF), induce accumulation of the GTP-bound form of p21ras. This accumulation could be caused either by an increase in guanine nucleotide exchange on p21ras or by a decrease in the GTPase activity of p21ras. To investigate whether insulin and EGF affect nucleotide exchange on p21ras, we measured binding of [alpha-32P]GTP to p21ras in cells permeabilized with streptolysin O. For this purpose, we used a cell line which expressed elevated levels of p21 H-ras and which was highly responsive to insulin and EGF. Stimulation with insulin or EGF resulted in an increase in the rate of nucleotide binding to p21ras. To determine whether this increased binding rate is due to the activation of a guanine nucleotide exchange factor, we made use of the inhibitory properties of a dominant negative mutant of p21ras, p21ras (Asn-17). Activation of p21ras by insulin and EGF in intact cells was abolished in cells infected with a recombinant vaccinia virus expressing p21ras (Asn-17). In addition, the enhanced nucleotide binding to p21ras in response to insulin and EGF in permeabilized cells was blocked upon expression of p21ras (Asn-17). From these data, we conclude that the activation of a guanine nucleotide exchange factor is involved in insulin- and EGF-induced activation of p21ras.
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