ArticleJournal of clinical microbiology1993
Detection of Mycobacterium tuberculosis in clinical samples by two-step polymerase chain reaction and nonisotopic hybridization methods.
Article in Journal of clinical microbiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 115 citations in OpenAlex.
- Prospective clinical evaluation of Amplicor Mycobacterium tuberculosis PCR test as a screening method in a low-prevalence population.Journal of clinical microbiology · 1996Trial
- Single-tube nested PCR in the diagnosis of tuberculosis.Journal of clinical pathology · 1996Trial
- Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by Amplicor PCR.Journal of clinical microbiology · 1995Trial
- Rapid diagnosis of Argentine hemorrhagic fever by reverse transcriptase PCR-based assay.Journal of clinical microbiology · 1995Trial
- Advances in Directly Amplifying Nucleic Acids from Complex Samples.Biosensors · 2019Review
- XtracTB Assay, a Mycobacterium tuberculosis molecular screening test with sensitivity approaching culture.Scientific reports · 2017Article
- Rapid identification of Mycobacterium tuberculosis complex by a novel hybridization signal amplification method based on self-assembly of DNA-streptavidin nanoparticles.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- Comparison of in-house PCR with conventional techniques and Cobas Amplicor M. tuberculosis kit for detection of Mycobacterium tuberculosis.Yonsei medical journal · 2008Article
- Detection and identification of Mycobacterium species isolates by DNA microarray.Journal of clinical microbiology · 2003Article
- Use of multiepitope polyproteins in serodiagnosis of active tuberculosis.Clinical and diagnostic laboratory immunology · 2002Article
- Rapid detection of smear-negative Mycobacterium tuberculosis by PCR and sequencing for rifampin resistance with DNA extracted directly from slides.Journal of clinical microbiology · 2001Article
- Single-tube balanced heminested PCR for detecting Mycobacterium tuberculosis in smear-negative samples.Journal of clinical microbiology · 2000Article
- Detection of mycobacterial DNA in papulonecrotic tuberculid lesions by polymerase chain reaction.Journal of clinical laboratory analysis · 2000Article
- Evaluation of the Abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis complex in human samples.Journal of clinical microbiology · 1999Article
- Absence of the genetic marker IS6110 from a strain of Mycobacterium tuberculosis isolated in Ontario.The Canadian journal of infectious diseases = Journal canadien des maladies infectieuses · 1998Article
- Evaluation of a rapid air thermal cycler for detection of Mycobacterium tuberculosis.Journal of clinical microbiology · 1997Article
- Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory.Clinical microbiology reviews · 1997Review
- Specificity of IS6110-based amplification assays for Mycobacterium tuberculosis complex.Journal of clinical microbiology · 1996Article
- Direct identification and typing of Mycobacterium tuberculosis by PCR.Journal of clinical microbiology · 1996Article
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4 authors at 1 institution in 1 country.
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Abstract
Detection of Mycobacterium tuberculosis in clinical specimens by the polymerase chain reaction (PCR) was compared with detection by culture. A 317-bp segment within the M. tuberculosis-specific insertion sequence IS6110 was amplified. The detection limit of the PCR assay for cultured mycobacteria was 50 cells per reaction by ethidium bromide-stained agarose gel electrophoresis and 5 cells per reaction by hybridization with an oligonucleotide probe conjugated with either digoxigenin or alkaline phosphatase (AP). This sensitivity was reduced fivefold in sputum specimens seeded with M. tuberculosis. Seventy-six clinical specimens were amplified and examined by the three detection methods. Both the digoxigenin and AP procedures were found to be more sensitive than agarose gel electrophoresis, but they were occasionally associated with a high background. An additional 308 specimens were examined only by agarose gel electrophoresis and the AP procedure. Of 71 specimens found to contain M. tuberculosis, amplified products were detected from 56 (79%) samples by agarose gel electrophoresis and/or the AP procedure. Of the additional 313 specimens that were culture negative for M. tuberculosis, 19 (6%) had amplified products detectable by agarose gel electrophoresis and/or the AP procedure. Compared with culture, PCR showed sensitivities and specificities of 55 and 98%, respectively, for agarose gel electrophoresis and 74 and 95%, respectively, for the AP procedure. Despite this low sensitivity, a rapid positive PCR result was accurate and clinically useful.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.