ArticleThe Journal of cell biology1993
Golgi-derived vesicles from developing epithelial cells bind actin filaments and possess myosin-I as a cytoplasmically oriented peripheral membrane protein.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 189 citations in OpenAlex.
- Myosin-I molecular motors at a glance.Journal of cell science · 2016Review
- MIiSR: Molecular Interactions in Super-Resolution Imaging Enables the Analysis of Protein Interactions, Dynamics and Formation of Multi-protein Structures.PLoS computational biology · 2015Article
- Myo1c is an unconventional myosin required for zebrafish glomerular development.Kidney international · 2013Article
- Actin acting at the Golgi.Histochemistry and cell biology · 2013Review
- Myosin Ia is required for CFTR brush border membrane trafficking and ion transport in the mouse small intestine.Traffic (Copenhagen, Denmark) · 2012Article
- Motor protein Myo1c is a podocyte protein that facilitates the transport of slit diaphragm protein Neph1 to the podocyte membrane.Molecular and cellular biology · 2011Article
- Differential localization and dynamics of class I myosins in the enterocyte microvillus.Molecular biology of the cell · 2010Article
- Apical trafficking in epithelial cells: signals, clusters and motors.Journal of cell science · 2009Review
- The unique architecture of Bunyamwera virus factories around the Golgi complex.Cellular microbiology · 2008Article
- Anomalous surface distribution of glycosyl phosphatidyl inositol-anchored proteins in neurons lacking acid sphingomyelinase.Molecular biology of the cell · 2008Article
- Neuronal membrane cholesterol loss enhances amyloid peptide generation.The Journal of cell biology · 2004Article
- Loss of myosin VI reduces secretion and the size of the Golgi in fibroblasts from Snell's waltzer mice.The EMBO journal · 2003Article
- Myosin motors and not actin comets are mediators of the actin-based Golgi-to-endoplasmic reticulum protein transport.Molecular biology of the cell · 2003Article
- Loss of the F-actin binding and vesicle-associated protein comitin leads to a phagocytosis defect.Eukaryotic cell · 2002Article
- Actin and pollen tube growth.Protoplasma · 2001Review
- Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells.Biophysical journal · 2000Article
- The golgi-associated COPI-coated buds and vesicles contain beta/gamma -actin.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- VIP17/MAL, a lipid raft-associated protein, is involved in apical transport in MDCK cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Kinetic analysis of secretory protein traffic and characterization of golgi to plasma membrane transport intermediates in living cells.The Journal of cell biology · 1998Article
- The localization of myosin VI at the golgi complex and leading edge of fibroblasts and its phosphorylation and recruitment into membrane ruffles of A431 cells after growth factor stimulation.The Journal of cell biology · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In the intestinal brush border, the mechanoenzyme myosin-I links the microvillus core actin filaments with the plasma membrane. Previous immunolocalization shows that myosin-I is associated with vesicles in mature enterocytes (Drenckhahn, D., and R. Dermietzel. 1988. J. Cell Biol. 107:1037-1048) suggesting a potential role mediating vesicle motility. We now report that myosin-I is associated with Golgi-derived vesicles isolated from cells that are rapidly assembling brush borders in intestinal crypts. Crypt cells were isolated in hyperosmotic buffer, homogenized, and fractionated using differential- and equilibrium-density centrifugation. Fractions containing 50-100-nm vesicles, a similar size to those observed in situ, were identified by EM and were shown to contain myosin-I as demonstrated by immunoblotting and immunolabel negative staining. Galactosyltransferase, a marker enzyme for trans-Golgi membranes was present in these fractions, as was alkaline phosphatase, which is an apical membrane targeted enzyme. Galactosyltransferase was also present in vesicles immuno-purified with antibodies to myosin-I. Villin, a marker for potential contamination from fragmented microvilli, was absent. Myosin-I was found to reside on the vesicle "outer" or cytoplasmic surface for it was accessible to exogenous proteases and intact vesicles could be immunolabeled with myosin-I antibodies in solution. The bound myosin-I could be extracted from the vesicles using NaCl, KI and Na2CO3, suggesting that it is a vesicle peripheral membrane protein. These vesicles were shown to bundle actin filaments in an ATP-dependent manner. These results are consistent with a role for myosin-I as an apically targeted motor for vesicle translocation in epithelial cells.
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