ArticleThe Journal of cell biology1993
Identification of intermediates in the pathway of protein import into chloroplasts and their localization to envelope contact sites.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 178 citations in OpenAlex.
- The journey of preproteins across the chloroplast membrane systems.Frontiers in physiology · 2023Review
- Origins, function, and regulation of the TOC-TIC general protein import machinery of plastids.Journal of experimental botany · 2020Article
- En route into chloroplasts: preproteins' way home.Photosynthesis research · 2018Review
- Lipid transport required to make lipids of photosynthetic membranes.Photosynthesis research · 2018Review
- Effects of TROL Presequence Mutagenesis on Its Import and Dual Localization in Chloroplasts.International journal of molecular sciences · 2018Article
- Import of Soluble Proteins into Chloroplasts and Potential Regulatory Mechanisms.Frontiers in plant science · 2017Review
- New insights into the mechanism of chloroplast protein import and its integration with protein quality control, organelle biogenesis and development.Journal of molecular biology · 2015Review
- Structural determinants allowing transferase activity in SENSITIVE TO FREEZING 2, classified as a family I glycosyl hydrolase.The Journal of biological chemistry · 2014Article
- Spatial association with PTEX complexes defines regions for effector export into Plasmodium falciparum-infected erythrocytes.Nature communications · 2013Article
- Tic22 is an essential chaperone required for protein import into the apicoplast.The Journal of biological chemistry · 2012Article
- The molecular basis for distinct pathways for protein import into Arabidopsis chloroplasts.The Plant cell · 2010Article
- Toc receptor dimerization participates in the initiation of membrane translocation during protein import into chloroplasts.The Journal of biological chemistry · 2009Article
- Interaction of actin and the chloroplast protein import apparatus.The Journal of biological chemistry · 2009Article
- A 1-megadalton translocation complex containing Tic20 and Tic21 mediates chloroplast protein import at the inner envelope membrane.The Plant cell · 2009Article
- The role of GTP binding and hydrolysis at the atToc159 preprotein receptor during protein import into chloroplasts.The Journal of cell biology · 2008Article
- Evidence for a dynamic and transient pathway through the TAT protein transport machinery.The EMBO journal · 2007Article
- Arabidopsis Cor15am is a chloroplast stromal protein that has cryoprotective activity and forms oligomers.Plant physiology · 2007Article
- Reconstitution of protein targeting to the inner envelope membrane of chloroplasts.The Journal of cell biology · 2006Article
- The plastid division proteins, FtsZ1 and FtsZ2, differ in their biochemical properties and sub-plastidial localization.The Biochemical journal · 2005Article
- Members of the Toc159 import receptor family represent distinct pathways for protein targeting to plastids.Molecular biology of the cell · 2004Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used a hybrid precursor protein to study the pathway of protein import into chloroplasts. This hybrid (pS/protA) consists of the precursor to the small subunit of Rubisco (pS) fused to the IgG binding domains of staphylococcal protein A. The pS/protA is efficiently imported into isolated chloroplasts and is processed to its mature form (S/protA). In addition to the mature stromal form, two intermediates in the pathway of pS/protA import were identified at early time points in the import reaction. The first intermediate represents unprocessed pS/protA bound to the outer surface of the chloroplast envelope and is analogous to a previously characterized form of pS that is specifically bound to the chloroplast surface and can be subsequently translocated in the stroma (Cline, K., M. Werner-Washburne, T. H. Lubben, and K. Keegstra. 1985. J. Biol. Chem. 260:3691-3696.) The second intermediate represents a partially translocated form of the precursor that remains associated with the envelope membrane. This form is processed to mature S/protA, but remains susceptible to exogenously added protease in intact chloroplasts. We conclude that the envelope associated S/protA is spanning both the outer and inner chloroplast membranes en route to the stroma. Biochemical and immunochemical localization of the two translocation intermediates indicates that both forms are exposed at the surface of the outer membrane at sites where the outer and inner membrane are closely apposed. These contact zones appear to be organized in a reticular network on the outer envelope. We propose a model for protein import into chloroplasts that has as its central features two distinct protein conducting channels in the outer and inner envelope membranes, each gated open by a distinct subdomain of the pS signal sequence.
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