ArticleProceedings of the National Academy of Sciences of the United States of America1993
The disaggregation theory of signal transduction revisited: further evidence that G proteins are multimeric and disaggregate to monomers when activated.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 60 citations in OpenAlex.
- Ligand-Induced Coupling between Oligomers of the MBiophysical journal · 2018Article
- Direct Coupling of a Seven-Transmembrane-Span Receptor to a Gαi G-Protein Regulatory Motif Complex.Molecular pharmacology · 2015Article
- Regulation of the AGS3·G{alpha}i signaling complex by a seven-transmembrane span receptor.The Journal of biological chemistry · 2010Article
- FGF acts as a co-transmitter through adenosine A(2A) receptor to regulate synaptic plasticity.Nature neuroscience · 2008Article
- Oligomerization of neuropeptide Y (NPY) Y2 receptors in CHO cells depends on functional pertussis toxin-sensitive G-proteins.Regulatory peptides · 2007Article
- Oligomerization of G protein-coupled receptors: past, present, and future.Biochemistry · 2004Review
- Article
- Functional rescue of a constitutively desensitized beta2AR through receptor dimerization.The Biochemical journal · 1998Article
- Analysis of the N-terminal binding domain of Go alpha.The Biochemical journal · 1997Article
- Is signal transduction modulated by an interaction between heterotrimeric G-proteins and tubulin?Endocrine · 1997Review
- N-terminal binding domain of Galpha subunits: involvement of amino acids 11-14 of Galphao in membrane attachment.The Biochemical journal · 1997Article
- Distinct biochemical properties of the native members of the G12 G-protein subfamily. Characterization of G alpha 12 purified from rat brain.The Biochemical journal · 1996Article
- Effect of colchicine and taxol on thyrotropin-releasing hormone receptor coupling to G protein in GH(3) cells.Endocrine · 1996Article
- The photoactivatable NAD+ analogue [32P]2-azido-NAD+ defines intra- and inter-molecular interactions of the C-terminal domain of the G-protein G alpha t.The Biochemical journal · 1995Article
- Oligomeric structure of caveolin: implications for caveolae membrane organization.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Immunohistochemical localization of adenylyl cyclase in rat brain indicates a highly selective concentration at synapses.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Close association of the alpha subunits of Gq and G11 G proteins with actin filaments in WRK1 cells: relation to G protein-mediated phospholipase C activation.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Signal transduction: evolution of an idea.Environmental health perspectives · 1995Review
- Characterization of caveolin-rich membrane domains isolated from an endothelial-rich source: implications for human disease.The Journal of cell biology · 1994Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have compared the sedimentation rates on sucrose gradients of the heterotrimeric GTP-binding regulatory (G) proteins Gs, G(o), Gi, and Gq extracted from rat brain synaptoneurosomes with Lubrol and digitonin. The individual alpha and beta subunits were monitored with specific antisera. In all cases, both subunits cosedimented, indicating that the subunits are likely complexed as heterotrimers. When extracted with Lubrol all of the G proteins sedimented with rates of about 4.5 S (consistent with heterotrimers) whereas digitonin extracted 60% of the G proteins with peaks at 11 S; 40% pelleted as larger structures. Digitonin-extracted Gi was cross-linked by p-phenylenedimaleimide, yielding structures too large to enter polyacrylamide gels. No cross-linking of Lubrol-extracted Gi occurred. Treatment of the membranes with guanosine 5'-[gamma-thio]triphosphate and Mg2+ yielded digitonin-extracted structures with peak sedimentation values of 8.5 S--i.e., comparable to that of purified G(o) in digitonin and considerably larger than the Lubrol-extracted 2S structures representing the separated alpha and beta gamma subunits formed by the actions of guanosine 5'-[gamma-thio]triphosphate. It is concluded that the multimeric structures of G proteins in brain membranes are at least partially preserved in digitonin and that activation of these structures in membranes yields monomers of G proteins rather than the disaggregated products (alpha and beta gamma complexes) observed in Lubrol. It is proposed that hormones and GTP affect the dynamic interplay between multimeric G proteins and receptors in a fashion analogous to the actions of ATP on the dynamic interactions between myosin and actin filaments. Signal transduction is mediated by activated monomers released from the multimers during the activation process.
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