ArticlePflugers Archiv : European journal of physiology1993
Effect of short-chain fatty acids on cell volume and intracellular pH in rat distal colon.
Article in Pflugers Archiv : European journal of physiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 54 citations in OpenAlex.
- Amino acid metabolism by pig intestinal bacteria and consequences for intestinal health and environment: current knowledge and future directions.Journal of animal science and biotechnology · 2026Review
- Involvement of butyrate in electrogenic KPflugers Archiv : European journal of physiology · 2019Article
- Essential Oils as an Intervention Strategy to ReduceFrontiers in microbiology · 2019Review
- Organic Acids and Potential for Modifying the Avian Gastrointestinal Tract and Reducing Pathogens and Disease.Frontiers in veterinary science · 2018Review
- Ischemic post-conditioning to counteract intestinal ischemia/reperfusion injury.World journal of gastrointestinal pathophysiology · 2010Article
- Short chain fatty acids differentially modulate cellular phenotype and c-myc protein levels in primary human nonmalignant and malignant colonocytes.Digestive diseases and sciences · 2001Article
- Fluid absorption in isolated perfused colonic crypts.The Journal of clinical investigation · 1995Article
- An Na(+)-independent short-chain fatty acid transporter contributes to intracellular pH regulation in murine colonocytes.The Journal of general physiology · 1995Article
- Extracellular pH regulation in microdomains of colonic crypts: effects of short-chain fatty acids.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Colonic fermentation: metabolic and clinical implications.The Clinical investigator · 1994Article
- The effect of short-chain fatty acids on Cl- and K+ conductance in rat colonic crypts.Pflugers Archiv : European journal of physiology · 1994Article
- Segmental differences along the crypt axis in the response of cell volume to secretagogues or hypotonic medium in the rat colon.Pflugers Archiv : European journal of physiology · 1994Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Superfusion of isolated crypts from the rat colon with sodium-butyrate-containing solutions induced an increase in the crypt diameter indicating a swelling of the crypt cells. The response to butyrate (50 mmol l-1) was not uniform along the crypt axis, the most pronounced swelling being observed in the upper third of the crypt. The butyrate effect was concentration-dependent and was completely suppressed by amiloride, suggesting that it is caused by activation of the Na+/H+ exchanger. Acetate, propionate and isobutyrate had a similar action. In HEPES-buffered solution the butyrate-induced change in cell volume was monophasic, i. e. only a swelling took place, whereas in HCO3- buffer it was biphasic, i. e. swelling was followed by a regulatory volume decrease. This decrease was suppressed by K+ and Cl- channel blockers as well as inhibitors of leukotriene synthesis. Measurements of intracellular pH with the fluorescent dye 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) revealed that butyrate induced an acidification of the cell, which was stronger in HEPES than in HCO3- buffer. Estimation of Na+/H+ exchange activity, tested as recovery of intracellular pH from an acid load via an NH4Cl prepulse, revealed a much lower Na+/H+ exchange activity in the fundus region compared to the upper third of the crypt. The smaller volume response evoked by butyrate in the fundus region probably reflects the smaller Na+/H+ activity compared to the more differentiated cells near the opening of the crypt. It is concluded that cell swelling caused by short-chain fatty acids is a physiological stimulus for volume regulation. This response is restricted to the more differentiated cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.