ArticleMolecular and cellular biology1993
Homologous and illegitimate recombination in developing Xenopus oocytes and eggs.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Translesion DNA synthesis-driven mutagenesis in very early embryogenesis of fast cleaving embryos.Nucleic acids research · 2022Article
- Ensemble and Single-Molecule Analysis of Non-Homologous End Joining in Frog Egg Extracts.Methods in enzymology · 2017Article
- Genome Engineering with TALE and CRISPR Systems in Neuroscience.Frontiers in genetics · 2016Review
- Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer.PloS one · 2013Article
- Mechanistic analysis of Xenopus EXO1's function in 5'-strand resection at DNA double-strand breaks.Nucleic acids research · 2011Article
- Efficiency of nonhomologous DNA end joining varies among somatic tissues, despite similarity in mechanism.Cellular and molecular life sciences : CMLS · 2011Article
- Nonhomologous DNA end joining in cell-free extracts.Journal of nucleic acids · 2010Article
- Article
- A novel cell-free system reveals a mechanism of circular DNA formation from tandem repeats.Nucleic acids research · 2001Article
- Hemicatenanes form upon inhibition of DNA replication.Nucleic acids research · 2000Article
- DNA damage-inducible and RAD52-independent repair of DNA double-strand breaks in Saccharomyces cerevisiae.Genetics · 2000Article
- Regulated formation of extrachromosomal circular DNA molecules during development in Xenopus laevis.Molecular and cellular biology · 1999Article
- Ku-dependent nonhomologous DNA end joining in Xenopus egg extracts.Molecular and cellular biology · 1999Article
- Nonhomologous end joining during restriction enzyme-mediated DNA integration in Saccharomyces cerevisiae.Molecular and cellular biology · 1998Article
- Non-homologous DNA end joining in plant cells is associated with deletions and filler DNA insertions.Nucleic acids research · 1997Article
- Cell cycle and genetic requirements of two pathways of nonhomologous end-joining repair of double-strand breaks in Saccharomyces cerevisiae.Molecular and cellular biology · 1996Article
- Endonuclease-induced, targeted homologous extrachromosomal recombination in Xenopus oocytes.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Nonhomologous DNA end joining in Schizosaccharomyces pombe efficiently eliminates DNA double-strand-breaks from haploid sequences.Nucleic acids research · 1994Article
- Nonhomologous DNA end joining of synthetic hairpin substrates in Xenopus laevis egg extracts.Nucleic acids research · 1994Article
- Illegitimate recombination in Xenopus: characterization of end-joined junctions.Nucleic acids research · 1994Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Exogenous DNA is efficiently recombined when injected into the nuclei of Xenopus laevis oocytes. This reaction proceeds by a homologous resection-annealing mechanism which depends on the activity of a 5'-->3' exonuclease. Two possible functions for this recombination activity have been proposed: it may be a remnant of an early process in oogenesis, such as meiotic recombination or amplification of genes coding for rRNA, or it may reflect materials stored for embryogenesis. To test these hypotheses, recombination capabilities were examined with oocytes at various developmental stages. Late-stage oocytes performed only homologous recombination, whereas the smallest oocytes ligated the restriction ends of the injected DNA but supported no homologous recombination. This transition from ligation to recombination activity was also seen in nuclear extracts from these same stages. Exonuclease activity was measured in the nuclear extracts and found to be low in early stages and then to increase in parallel with recombination capacity in later stages. The accumulation of exonuclease and recombination activities during oogenesis suggests that they are stored for embryogenesis and are not present for oocyte-specific functions. Eggs were also tested and found to catalyze homologous recombination, ligation, and illegitimate recombination. Retention of homologous recombination in eggs is consistent with an embryonic function for the resection-annealing mechanism. The observation of all three reactions in eggs suggests that multiple pathways are available for the repair of double-strand breaks during the extremely rapid cleavage stages after fertilization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.