ArticleMolecular and cellular biology1993
Short artificial hairpins sequester splicing signals and inhibit yeast pre-mRNA splicing.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Control of gene output by intron RNA structure.bioRxiv : the preprint server for biology · 2025Article
- CAR-SPLASH identifies nascent pre-mRNA structures implicated in kinetic coupling and alternative splicing.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- From benign to pathogenic variants and vice versa: pyrimidine transitions at position -3 of TAG and CAG 3' splice sites.Journal of human genetics · 2025Article
- Impact on splicing inRNA (New York, N.Y.) · 2023Article
- Pre-mRNA splicing and its cotranscriptional connections.Trends in genetics : TIG · 2023Review
- Conserved long-range base pairings are associated with pre-mRNA processing of human genes.Nature communications · 2021Article
- RNAs in the spliceosome: Insight from cryoEM structures.Wiley interdisciplinary reviews. RNA · 2019Review
- Splicing and transcription touch base: co-transcriptional spliceosome assembly and function.Nature reviews. Molecular cell biology · 2017Review
- The effects of structure on pre-mRNA processing and stability.Methods (San Diego, Calif.) · 2017Review
- Knockdown and replacement therapy mediated by artificial mirtrons in spinocerebellar ataxia 7.Nucleic acids research · 2017Article
- Identification of alternatively spliced transcripts of rice phytochelatin synthase 2 gene OsPCS2 involved in mitigation of cadmium and arsenic stresses.Plant molecular biology · 2017Article
- Reducing persistent polyomavirus infection increases functionality of virus-specific memory CD8 T cells.Virology · 2017Article
- RNA structure in splicing: An evolutionary perspective.RNA biology · 2016Review
- A Phylogenetic Survey on the Structure of the HIV-1 Leader RNA Domain That Encodes the Splice Donor Signal.Viruses · 2016Article
- Identification of cis-acting elements and splicing factors involved in the regulation of BIM Pre-mRNA splicing.PloS one · 2014Article
- RNA secondary structure mediates alternative 3'ss selection in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2012Article
- Role of RNA structure in regulating pre-mRNA splicing.Trends in biochemical sciences · 2010Review
- Limited complementarity between U1 snRNA and a retroviral 5' splice site permits its attenuation via RNA secondary structure.Nucleic acids research · 2009Article
- Alternative splicing as a therapeutic target for human diseases.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Widespread impact of nonsense-mediated mRNA decay on the yeast intronome.Molecular cell · 2008Article
Corrections and comments
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3 authors.
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Abstract
To examine the stability of yeast (Saccharomyces cerevisiae) pre-mRNA structures, we inserted a series of small sequence elements that generated potential RNA hairpins at the 5' splice site and branch point regions. We analyzed spliceosome assembly and splicing in vitro as well as splicing and nuclear pre-mRNA retention in vivo. Surprisingly, the inhibition of in vivo splicing approximately paralleled that of in vitro splicing. Even a 6-nucleotide hairpin could be shown to inhibit splicing, and a 15-nucleotide hairpin gave rise to almost complete inhibition. The in vitro results indicate that hairpins that sequester the 5' splice site have a major effect on the early steps of spliceosome assembly, including U1 small nuclear ribonucleoprotein binding. The in vivo experiments lead to comparable conclusions as the sequestering hairpins apparently result in the transport of pre-mRNA to the cytoplasm. The observations are compared with previous data from both yeast and mammalian systems and suggest an important effect of pre-mRNA structure on in vivo splicing.
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