ArticleMolecular and cellular biology1993
The major histocompatibility complex class II promoter-binding protein RFX (NF-X) is a methylated DNA-binding protein.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- c-Jun/c-Fos heterodimers regulate cellular genes via a newly identified class of methylated DNA sequence motifs.Nucleic acids research · 2014Article
- A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein.PloS one · 2011Article
- Article
- Sequence-specific recognition of methylated DNA by human zinc-finger proteins.Nucleic acids research · 2010Article
- The Igf2/H19 imprinting control region exhibits sequence-specific and cell-type-dependent DNA methylation-mediated repression.Nucleic acids research · 2009Article
- Collagen alpha1(I) gene (COL1A1) is repressed by RFX family.The Journal of biological chemistry · 2005Article
- Methylation of the asparagine synthetase promoter in human leukemic cell lines is associated with a specific methyl binding protein.Oncogene · 2004Article
- Hypermethylation of the spleen tyrosine kinase promoter in T-lineage acute lymphoblastic leukemia.Oncogene · 2003Article
- Common methylation characteristics of sex chromosomes in somatic and germ cells from mouse, lemur and human.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2000Article
- The transcriptional activation and repression domains of RFX1, a context-dependent regulator, can mutually neutralize their activities.Nucleic acids research · 1997Article
- Sequence-independent inhibition of RNA transcription by DNA dumbbells and other decoys.Nucleic acids research · 1997Article
- Interactions of the transcription factors MIBP1 and RFX1 with the EP element of the hepatitis B virus enhancer.Journal of virology · 1996Article
- Complex architecture of major histocompatibility complex class II promoters: reiterated motifs and conserved protein-protein interactions.Molecular and cellular biology · 1996Article
- A consensus motif in the RFX DNA binding domain and binding domain mutants with altered specificity.Molecular and cellular biology · 1996Article
- Multiple single-stranded cis elements are associated with activated chromatin of the human c-myc gene in vivo.Molecular and cellular biology · 1996Article
- Increasing binding of a transcription factor immediately downstream of the cap site of a cytomegalovirus gene represses expression.Nucleic acids research · 1995Article
- The myc intron-binding polypeptide associates with RFX1 in vivo and binds to the major histocompatibility complex class II promoter region, to the hepatitis B virus enhancer, and to regulatory regions of several distinct viral genes.Molecular and cellular biology · 1995Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A mammalian protein called RFX or NF-X binds to the X box (or X1 box) in the promoters of a number of major histocompatibility (MHC) class II genes. In this study, RFX was shown to have the same DNA-binding specificity as methylated DNA-binding protein (MDBP), and its own cDNA was found to contain a binding site for MDBP in the leader region. MDBP is a ubiquitous mammalian protein that binds to certain DNA sequences preferentially when they are CpG methylated and to other related sequences, like the X box, irrespective of DNA methylation. MDBP from HeLa and Raji cells formed DNA-protein complexes with X-box oligonucleotides that coelectrophoresed with those containing standard MDBP sites. Furthermore, MDBP and X-box oligonucleotides cross-competed for the formation of these DNA-protein complexes. DNA-protein complexes obtained with MDBP sites displayed the same partial supershifting with an antiserum directed to the N terminus of RFX seen for complexes containing an X-box oligonucleotide. Also, the in vitro-transcribed-translated product of a recombinant RFX cDNA bound specifically to MDBP ligands and displayed the DNA methylation-dependent binding of MDBP. RFX therefore contains MDBP activity and thereby also EF-C, EP, and MIF activities that are indistinguishable from MDBP and that bind to methylation-independent sites in the transcriptional enhancers of polyomavirus and hepatitis B virus and to an intron of c-myc.
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