ArticleMolecular and cellular biology1993
Binding of a phosphoprotein to the 3' untranslated region of the mouse protamine 2 mRNA temporally represses its translation.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 157 citations in OpenAlex.
- Bovine Spermatogenesis.Advances in anatomy, embryology, and cell biology · 2025Review
- Biochemical characterization of clinically relevant mutations of human Translin.Molecular and cellular biochemistry · 2023Article
- A transcriptional biosensor to monitor single cancer cell therapeutic responses by bioluminescence microscopy.Theranostics · 2022Article
- Functional characterization of testis-brain RNA-binding protein, TB-RBP/Translin, in translational regulation.The Journal of reproduction and development · 2021Article
- Article
- Translin: A multifunctional protein involved in nucleic acid metabolism.Journal of biosciences · 2019Review
- Learning induces the translin/trax RNase complex to express activin receptors for persistent memory.eLife · 2017Article
- Chromatin and extracellular vesicle associated sperm RNAs.Nucleic acids research · 2015Article
- The transacting factor CBF-A/Hnrnpab binds to the A2RE/RTS element of protamine 2 mRNA and contributes to its translational regulation during mouse spermatogenesis.PLoS genetics · 2013Article
- Low-resolution structure of Drosophila translin.FEBS open bio · 2012Article
- The sperm nucleus: chromatin, RNA, and the nuclear matrix.Reproduction (Cambridge, England) · 2011Review
- Dendritic trafficking of BDNF mRNA is mediated by translin and blocked by the G196A (Val66Met) mutation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Behavioral and neurochemical alterations in mice lacking the RNA-binding protein translin.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- RNA transport and local control of translation.Annual review of cell and developmental biology · 2005Review
- Mice deficient for testis-brain RNA-binding protein exhibit a coordinate loss of TRAX, reduced fertility, altered gene expression in the brain, and behavioral changes.Molecular and cellular biology · 2003Article
- Candidate RNA-binding proteins regulating extrasomatic mRNA targeting and translation in mammalian neurons.Molecular neurobiology · 2002Review
- Translational control of human p53 expression in yeast mediated by 5'-UTR-ORF structural interaction.Nucleic acids research · 2001Article
- Sequence-independent assembly of spermatid mRNAs into messenger ribonucleoprotein particles.Molecular and cellular biology · 1999Article
- Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes.Genes & development · 1998Article
- Localization-dependent translation requires a functional interaction between the 5' and 3' ends of oskar mRNA.Genes & development · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The synthesis of the protamines, the predominant nuclear proteins of mammalian spermatozoa, is regulated during germ cell development by mRNA storage for about 7 days in the cytoplasm of differentiating spermatids. Two highly conserved sequences, the Y and H elements present in the 3' untranslated regions (UTRs) of all known mammalian protamine mRNAs, form RNA-protein complexes and specifically bind a protein of 18 kDa. Here, we show that translation of fusion mRNAs was markedly repressed in reticulocyte lysates supplemented with a mouse testis extract enriched for the 18-kDa protein when the mRNAs contained the 3' UTR of mouse protamine 2 (mP2) or the Y and H elements of mP2. No significant decrease was seen when the fusion mRNAs contained the 3' UTR of human growth hormone. The 18-kDa protein is developmentally regulated in male germ cells, requires phosphorylation for RNA binding, and is found in the ribonucleoprotein particle fractions of a testicular postmitochondrial supernatant. We propose that a phosphorylated 18-kDa protein plays a primary role in repressing translation of mP2 mRNA by interaction with the highly conserved Y and H elements. At a later stage of male gamete differentiation, the 18-kDa protein no longer binds to the mRNA, likely as a result of dephosphorylation, enabling the protamine mRNA to be translated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.