ArticleMolecular and cellular biology1993
Human ERCC5 cDNA-cosmid complementation for excision repair and bipartite amino acid domains conserved with RAD proteins of Saccharomyces cerevisiae and Schizosaccharomyces pombe.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 51 citations in OpenAlex.
- Global contributions to the understanding of DNA repair and skin cancer.The Journal of investigative dermatology · 2014Review
- Stochastic and reversible assembly of a multiprotein DNA repair complex ensures accurate target site recognition and efficient repair.The Journal of cell biology · 2010Article
- XPG: its products and biological roles.Advances in experimental medicine and biology · 2008Review
- Recruitment of the nucleotide excision repair endonuclease XPG to sites of UV-induced dna damage depends on functional TFIIH.Molecular and cellular biology · 2006Article
- The human XPG gene: gene architecture, alternative splicing and single nucleotide polymorphisms.Nucleic acids research · 2001Article
- Comparison of the 5' nuclease activities of taq DNA polymerase and its isolated nuclease domain.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Transcription-coupled repair of DNA damage: unanticipated players, unexpected complexities.American journal of human genetics · 1999Review
- A structure-specific endonuclease from cauliflower (Brassica oleracea var. botrytis) inflorescence.Nucleic acids research · 1997Article
- A common mutational pattern in Cockayne syndrome patients from xeroderma pigmentosum group G: implications for a second XPG function.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Open complex formation around a lesion during nucleotide excision repair provides a structure for cleavage by human XPG protein.The EMBO journal · 1997Article
- ERCC4 (XPF) encodes a human nucleotide excision repair protein with eukaryotic recombination homologs.Molecular and cellular biology · 1996Article
- Molecular cloning and structural analysis of the functional mouse genomic XPG gene.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Ultraviolet-induced movement of the human DNA repair protein, Xeroderma pigmentosum type G, in the nucleus.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- An XPG DNA repair defect causing mutagen hypersensitivity in mouse leukemia L1210 cells.Molecular and cellular biology · 1995Article
- Nucleotide excision repair in yeast.Current genetics · 1994Review
- Structural and functional conservation of the human homolog of the Schizosaccharomyces pombe rad2 gene, which is required for chromosome segregation and recovery from DNA damage.Molecular and cellular biology · 1994Article
- The rad16 gene of Schizosaccharomyces pombe: a homolog of the RAD1 gene of Saccharomyces cerevisiae.Molecular and cellular biology · 1994Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several human genes related to DNA excision repair (ER) have been isolated via ER cross-species complementation (ERCC) of UV-sensitive CHO cells. We have now isolated and characterized cDNAs for the human ERCC5 gene that complement CHO UV135 cells. The ERCC5 mRNA size is about 4.6 kb. Our available cDNA clones are partial length, and no single clone was active for UV135 complementation. When cDNAs were mixed pairwise with a cosmid clone containing an overlapping 5'-end segment of the ERCC5 gene, DNA transfer produced UV-resistant colonies with 60 to 95% correction of UV resistance relative to either a genomic ERCC5 DNA transformant or the CHO AA8 progenitor cells. cDNA-cosmid transformants regained intermediate levels (20 to 45%) of ER-dependent reactivation of a UV-damaged pSVCATgpt reporter plasmid. Our evidence strongly implicates an in situ recombination mechanism in cDNA-cosmid complementation for ER. The complete deduced amino acid sequence of ERCC5 was reconstructed from several cDNA clones encoding a predicted protein of 1,186 amino acids. The ERCC5 protein has extensive sequence similarities, in bipartite domains A and B, to products of RAD repair genes of two yeasts, Saccharomyces cerevisiae RAD2 and Schizosaccharomyces pombe rad13. Sequence, structural, and functional data taken together indicate that ERCC5 and its relatives are probable functional homologs. A second locus represented by S. cerevisiae YKL510 and S. pombe rad2 genes is structurally distinct from the ERCC5 locus but retains vestigial A and B domain similarities. Our analyses suggest that ERCC5 is a nuclear-localized protein with one or more highly conserved helix-loop-helix segments within domains A and B.
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