ArticleMolecular and cellular biology1993
A V(D)J recombinase-inducible B-cell line: role of transcriptional enhancer elements in directing V(D)J recombination.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 82 citations in OpenAlex.
- Access Guide to Antigen Receptor Genes.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- A Novel Quantitative Fluorescent Reporter Assay for RAG Targets and RAG Activity.Frontiers in immunology · 2013Article
- Requirement for enhancer specificity in immunoglobulin heavy chain locus regulation.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Transposon mutagenesis identifies sites upstream of the Neisseria gonorrhoeae pilE gene that modulate pilin antigenic variation.Journal of bacteriology · 2007Article
- Transcription and double-strand breaks induce similar mitotic recombination events in Saccharomyces cerevisiae.Genetics · 2002Article
- Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Stepwise activation of the immunoglobulin mu heavy chain gene locus.The EMBO journal · 2001Article
- Hpr1 is preferentially required for transcription of either long or G+C-rich DNA sequences in Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
- Article
- A new hyperrecombination mutation identifies a novel yeast gene, THP1, connecting transcription elongation with mitotic recombination.Genetics · 2001Article
- Regulation of antigen receptor gene assembly in lymphocytes.Immunologic research · 2001Review
- A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae.The EMBO journal · 2000Article
- Both V(D)J recombination and radioresistance require DNA-PK kinase activity, though minimal levels suffice for V(D)J recombination.Nucleic acids research · 2000Article
- Regulation of V(D)J recombination by transcriptional promoters.Molecular and cellular biology · 1999Article
- Recombination and transcription of the endogenous Ig heavy chain locus is effected by the Ig heavy chain intronic enhancer core region in the absence of the matrix attachment regions.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- ETS-core binding factor: a common composite motif in antigen receptor gene enhancers.Molecular and cellular biology · 1998Article
- Enhancer control of local accessibility to V(D)J recombinase.Molecular and cellular biology · 1997Article
- Coordinate transcription and V(D)J recombination of the kappa immunoglobulin light-chain locus: NF-kappaB-dependent and -independent pathways of activation.Molecular and cellular biology · 1997Article
- V(D)J recombination frequency is affected by the sequence interposed between a pair of recombination signals: sequence comparison reveals a putative recombinational enhancer element.Nucleic acids research · 1997Article
- Context dependent transactivation domains activate the immunoglobulin mu heavy chain gene enhancer.The EMBO journal · 1996Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Rapid analysis of mechanisms that regulate V(D)J recombination has been hampered by the lack of appropriate cell systems that reproduce aspects of normal prelymphocyte physiology in which the recombinase is activated, accessible antigen receptor loci are rearranged, and rearrangement status is fixed by termination of recombinase expression. To generate such a system, we introduced heat shock-inducible V(D)J recombination-activating genes (RAG) 1 and 2 into a recombinationally inert B-cell line. Heat shock treatment of these cells rapidly induced high levels of RAG transcripts and RAG proteins that were accompanied by a parallel induction of V(D)J recombinase activity, strongly suggesting that RAG proteins have a primary role in V(D)J recombination. Within hours after induction, these cells began to rearrange chromosomally integrated V(D)J recombination substrates but only if the substrates contained an active transcriptional enhancer; substrates lacking an enhancer were not efficiently rearranged. Activities necessary to target integrated substrates for rearrangement were provided by two separate lymphoid-specific transcriptional enhancers, as well as an active nonlymphoid enhancer, unequivocally demonstrating that such elements enhance both transcription and V(D)J recombinational accessibility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.