ArticleMolecular and cellular biology1993
PUB1: a major yeast poly(A)+ RNA-binding protein.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Post-transcriptional control of fungal cell wall synthesis.Cell surface (Amsterdam, Netherlands) · 2022Article
- Review
- Article
- A New Method for Determining Structure Ensemble: Application to a RNA Binding Di-Domain Protein.Biophysical journal · 2016Article
- Harnessing natural sequence variation to dissect posttranscriptional regulatory networks in yeast.G3 (Bethesda, Md.) · 2014Article
- The association of a La module with the PABP-interacting motif PAM2 is a recurrent evolutionary process that led to the neofunctionalization of La-related proteins.RNA (New York, N.Y.) · 2013Article
- Global analysis of yeast mRNPs.Nature structural & molecular biology · 2013Article
- Pub1p C-terminal RRM domain interacts with Tif4631p through a conserved region neighbouring the Pab1p binding site.PloS one · 2011Article
- Dissecting the expression dynamics of RNA-binding proteins in posttranscriptional regulatory networks.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The pivotal roles of TIA proteins in 5' splice-site selection of alu exons and across evolution.PLoS genetics · 2009Article
- A novel class of mRNA-containing cytoplasmic granules are produced in response to UV-irradiation.Molecular biology of the cell · 2008Article
- Imp associates with squid and Hrp48 and contributes to localized expression of gurken in the oocyte.Molecular and cellular biology · 2006Article
- Global analysis of Pub1p targets reveals a coordinate control of gene expression through modulation of binding and stability.Molecular and cellular biology · 2005Article
- RBP45 and RBP47, two oligouridylate-specific hnRNP-like proteins interacting with poly(A)+ RNA in nuclei of plant cells.RNA (New York, N.Y.) · 2000Article
- Scp160p, a multiple KH-domain protein, is a component of mRNP complexes in yeast.Nucleic acids research · 2000Article
- RNA-binding proteins TIA-1 and TIAR link the phosphorylation of eIF-2 alpha to the assembly of mammalian stress granules.The Journal of cell biology · 1999Article
- The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human.The EMBO journal · 1999Article
- Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1.Molecular and cellular biology · 1996Article
- Cap-dependent and cap-independent translation by internal initiation of mRNAs in cell extracts prepared from Saccharomyces cerevisiae.Molecular and cellular biology · 1994Article
- GRSF-1: a poly(A)+ mRNA binding protein which interacts with a conserved G-rich element.Nucleic acids research · 1994Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of RNA polymerase II transcripts can be regulated at the posttranscriptional level by RNA-binding proteins. Although extensively characterized in metazoans, relatively few RNA-binding proteins have been characterized in the yeast Saccharomyces cerevisiae. Three major proteins are cross-linked by UV light to poly(A)+ RNA in living S. cerevisiae cells. These are the 72-kDa poly(A)-binding protein and proteins of 60 and 50 kDa (S.A. Adam, T.Y. Nakagawa, M.S. Swanson, T. Woodruff, and G. Dreyfuss, Mol. Cell. Biol. 6:2932-2943, 1986). Here, we describe the 60-kDa protein, one of the major poly(A)+ RNA-binding proteins in S. cerevisiae. This protein, PUB1 [for poly(U)-binding protein 1], was purified by affinity chromatography on immobilized poly(rU), and specific monoclonal antibodies to it were produced. UV cross-linking demonstrated that PUB1 is bound to poly(A)+ RNA (mRNA or pre-mRNA) in living cells, and it was detected primarily in the cytoplasm by indirect immunofluorescence. The gene for PUB1 was cloned and sequenced, and the sequence was found to predict a 51-kDa protein with three ribonucleoprotein consensus RNA-binding domains and three glutamine- and asparagine-rich auxiliary domains. This overall structure is remarkably similar to the structures of the Drosophila melanogaster elav gene product, the human neuronal antigen HuD, and the cytolytic lymphocyte protein TIA-1. Each of these proteins has an important role in development and differentiation, potentially by affecting RNA processing. PUB1 was found to be nonessential in S. cerevisiae by gene replacement; however, further genetic analysis should reveal important features of this class of RNA-binding proteins.
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