ArticleThe Journal of clinical investigation1993
Distinct mechanisms of epithelial adhesion for Candida albicans and Candida tropicalis. Identification of the participating ligands and development of inhibitory peptides.
Article in The Journal of clinical investigation, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 81 citations in OpenAlex.
- A Membrane-Targeted Photosensitizer Prevents Drug Resistance and Induces Immune Response in Treating Candidiasis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Review
- Fibronectin-, vitronectin- and laminin-binding proteins at the cell walls of Candida parapsilosis and Candida tropicalis pathogenic yeasts.BMC microbiology · 2015Article
- Antifungal activity of amphotericin B and voriconazole against the biofilms and biofilm-dispersed cells of Candida albicans employing a newly developed in vitro pharmacokinetic model.Annals of clinical microbiology and antimicrobials · 2015Article
- Interactions of Candida albicans with host epithelial surfaces.Journal of oral microbiology · 2013Review
- Striking a balance: fungal commensalism versus pathogenesis.Current opinion in microbiology · 2013Review
- Insights into Candida tropicalis nosocomial infections and virulence factors.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Review
- Examination of potential virulence factors of Candida tropicalis clinical isolates from hospitalized patients.Mycopathologia · 2010Article
- Review
- Prevalence of yeasts in beach sand at three bathing beaches in South Florida.Water research · 2007Article
- Salivary statherin peptide-binding epitopes of commensal and potentially infectious Actinomyces spp. delineated by a hybrid peptide construct.Infection and immunity · 2004Article
- A monoclonal antibody directed against a Candida albicans cell wall mannoprotein exerts three anti-C. albicans activities.Infection and immunity · 2003Article
- Involvement of fungal cell wall components in adhesion of Sporothrix schenckii to human fibronectin.Infection and immunity · 2001Article
- Adhesion of Aspergillus species to extracellular matrix proteins: evidence for involvement of negatively charged carbohydrates on the conidial surface.Infection and immunity · 2000Article
- Hemoglobin-induced binding of Candida albicans to the cell-binding domain of fibronectin is independent of the Arg-Gly-Asp sequence.Infection and immunity · 1998Article
- Cell wall and secreted proteins of Candida albicans: identification, function, and expression.Microbiology and molecular biology reviews : MMBR · 1998Review
- The effects of monoclonal antibodies against iC3b receptors in mice with experimentally induced disseminated candidiasis.Immunology · 1997Article
- Blood group glycolipids as epithelial cell receptors for Candida albicans.Infection and immunity · 1996Article
- Antifungal agents: chemotherapeutic targets and immunologic strategies.Antimicrobial agents and chemotherapy · 1996Review
- Cloning and expression of a gene encoding an integrin-like protein in Candida albicans.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The yeast Candida albicans is the leading cause of disseminated fungal infection in neonates, immunocompromised hosts, diabetics, and postoperative patients; Candida tropicalis is the second most frequent isolate. Because the integrin analogue in C. albicans shares antigenic, structural, and functional homologies with the beta 2-integrin subunits alpha M and alpha X, we investigated the role of integrin analogues in epithelial adhesion of C. albicans and C. tropicalis. On flow cytometry with the monoclonal antibody (mAb) OKM1, surface fluorescence was highest for C. albicans and significantly reduced for C. tropicalis (P < 0.001). However, adhesion to the human epithelial cell line HeLa S3 did not differ for these two candidal species: specific adhesion was highest for C. albicans at 44.0 +/- 1.8%, and only slightly lower for C. tropicalis at 38.8 +/- 3.6% (P = NS). The disparity between expression of the integrin analogue and epithelial adhesion suggested distinct mechanisms for this process in C. albicans versus C. tropicalis. Preincubation of C. albicans with anti-alpha M mAbs, with purified iC3b (the RGD ligand for the integrin analogue), or with 9-15-mer RGD peptides from iC3b all inhibited epithelial adhesion significantly (P < 0.001-0.04). Purified fibronectin or fibronectin-RGD peptides failed to block C. albicans adhesion. In contrast, epithelial adhesion of C. tropicalis was significantly inhibited by purified fibronectin and its RGD peptides (P < or = 0.021), but not by iC3b nor the iC3b-RGD peptides. Both iC3b and fibronectin were identified on the surface of epithelial cells after growth in serum-free medium. A polyclonal antibody to C3 inhibited C. albicans adhesion while a control antibody to fibronectin was ineffective; the converse was true for C. tropicalis. These results indicate that the pathogenic yeasts C. albicans and C. tropicalis recognize distinct RGD ligands present at the surface of the epithelial cell and that these interactions can be differentially inhibited by defined RGD peptides containing appropriate flanking sequences.
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