ArticleThe Journal of cell biology1993
MIF2 is required for mitotic spindle integrity during anaphase spindle elongation in Saccharomyces cerevisiae.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed, 106 citations in OpenAlex.
- Cell cycle dependent methylation of Dam1 contributes to kinetochore integrity and faithful chromosome segregation.PLoS genetics · 2025Article
- Misregulation of cell cycle-dependent methylation of budding yeast CENP-A contributes to chromosomal instability.Molecular biology of the cell · 2023Article
- Multi-site phosphorylation of yeast Mif2/CENP-C promotes inner kinetochore assembly.Current biology : CB · 2023Article
- Cell cycle-dependent association of polo kinase Cdc5 with CENP-A contributes to faithful chromosome segregation in budding yeast.Molecular biology of the cell · 2019Article
- A Cohesin-Based Partitioning Mechanism Revealed upon Transcriptional Inactivation of Centromere.PLoS genetics · 2016Article
- The dimerization domain of PfCENP-C is required for its functions as a centromere protein in human malaria parasite Plasmodium falciparum.Malaria journal · 2014Article
- The Mub1/Ubr2 ubiquitin ligase complex regulates the conserved Dsn1 kinetochore protein.PLoS genetics · 2013Article
- Scm3 is a centromeric nucleosome assembly factor.The Journal of biological chemistry · 2011Article
- Article
- Moonlighting proteins Hal3 and Vhs3 form a heteromeric PPCDC with Ykl088w in yeast CoA biosynthesis.Nature chemical biology · 2009Article
- Dissection of CENP-C-directed centromere and kinetochore assembly.Molecular biology of the cell · 2009Article
- The C-terminal domain of CENP-C displays multiple and critical functions for mammalian centromere formation.PloS one · 2009Article
- Structural and functional dissection of Mif2p, a conserved DNA-binding kinetochore protein.Molecular biology of the cell · 2008Article
- The Ulp2 SUMO protease is required for cell division following termination of the DNA damage checkpoint.Molecular and cellular biology · 2007Article
- CENP-C is involved in chromosome segregation, mitotic checkpoint function, and kinetochore assembly.Molecular biology of the cell · 2007Article
- Suppressor analysis of a histone defect identifies a new function for the hda1 complex in chromosome segregation.Genetics · 2006Article
- Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homolog.Genes & development · 2005Article
- Inner kinetochore of the pathogenic yeast Candida glabrata.Eukaryotic cell · 2004Article
- Adaptive evolution of centromere proteins in plants and animals.Journal of biology · 2004Article
- Centromere DNA, proteins and kinetochore assembly in vertebrate cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The function of the essential MIF2 gene in the Saccharomyces cerevisiae cell cycle was examined by overepressing or creating a deficit of MIF2 gene product. When MIF2 was overexpressed, chromosomes missegregated during mitosis and cells accumulated in the G2 and M phases of the cell cycle. Temperature sensitive mutants isolated by in vitro mutagenesis delayed cell cycle progression when grown at the restrictive temperature, accumulated as large budded cells that had completed DNA replication but not chromosome segregation, and lost viability as they passed through mitosis. Mutant cells also showed increased levels of mitotic chromosome loss, supersensitivity to the microtubule destabilizing drug MBC, and morphologically aberrant spindles. mif2 mutant spindles arrested development immediately before anaphase spindle elongation, and then frequently broke apart into two disconnected short half spindles with misoriented spindle pole bodies. These findings indicate that MIF2 is required for structural integrity of the spindle during anaphase spindle elongation. The deduced Mif2 protein sequence shared no extensive homologies with previously identified proteins but did contain a short region of homology to a motif involved in binding AT rich DNA by the Drosophila D1 and mammalian HMGI chromosomal proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.