ArticleThe Journal of cell biology1993
Molecular analysis of the INCENPs (inner centromere proteins): separate domains are required for association with microtubules during interphase and with the central spindle during anaphase.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 103 citations in OpenAlex.
- Article
- A new layer of regulation of chromosomal passenger complex (CPC) translocation in budding yeast.Molecular biology of the cell · 2023Article
- Highway to hell-thy meiotic divisions: Chromosome passenger complex functions driven by microtubules: CPC interactions with both the chromosomes and microtubules are important for spindle assembly and function: CPC interactions with both the chromosomes and microtubules are important for spindle assembly and function.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- ER/K-link-Leveraging a native protein linker to probe dynamic cellular interactions.Methods in enzymology · 2021Review
- Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1.Journal of cell science · 2019Article
- Correcting aberrant kinetochore microtubule attachments: a hidden regulation of Aurora B on microtubules.Current opinion in cell biology · 2019Review
- RecQL4-Aurora B kinase axis is essential for cellular proliferation, cell cycle progression, and mitotic integrity.Oncogenesis · 2018Article
- Dual recognition of chromatin and microtubules by INCENP is important for mitotic progression.The Journal of cell biology · 2017Article
- An engineered minimal chromosomal passenger complex reveals a role for INCENP/Sli15 spindle association in chromosome biorientation.The Journal of cell biology · 2017Article
- The Ins and Outs of Aurora B Inner Centromere Localization.Frontiers in cell and developmental biology · 2017Review
- The Inner Centromere Protein (INCENP) Coil Is a Single α-Helix (SAH) Domain That Binds Directly to Microtubules and Is Important for Chromosome Passenger Complex (CPC) Localization and Function in Mitosis.The Journal of biological chemistry · 2015Article
- Review
- Molecular basis for phosphospecific recognition of histone H3 tails by Survivin paralogues at inner centromeres.Molecular biology of the cell · 2012Article
- Functional analysis of the microtubule-interacting transcriptome.Molecular biology of the cell · 2011Article
- Role of Survivin in cytokinesis revealed by a separation-of-function allele.Molecular biology of the cell · 2011Article
- Ipl1/Aurora-dependent phosphorylation of Sli15/INCENP regulates CPC-spindle interaction to ensure proper microtubule dynamics.The Journal of cell biology · 2011Article
- Sensing centromere tension: Aurora B and the regulation of kinetochore function.Trends in cell biology · 2011Review
- Use of DT40 conditional-knockout cell lines to study chromosomal passenger protein function.Biochemical Society transactions · 2010Article
- Dual detection of chromosomes and microtubules by the chromosomal passenger complex drives spindle assembly.Developmental cell · 2010Article
- INCENP-aurora B interactions modulate kinase activity and chromosome passenger complex localization.The Journal of cell biology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
It has recently been proposed that mitotic chromosomes transport certain cytoskeletal proteins to the metaphase plate so that these proteins are able to subsequently participate in the assembly of the anaphase spindle and the cleavage furrow. To understand how such proteins accomplish their dual chromosomal: cytoskeletal role, we have begun a molecular and functional analysis of the inner centromere proteins (INCENPs), founder members of the class of "chromosome passenger proteins". cDNA clones encoding the open reading frames of the two chicken INCENPs were recovered. The predicted proteins, class I INCENP (96,357 D) and class II INCENP (100,931 D) are novel, and differ from each other by the inclusion of a 38-codon insert within the class II coding region. Transient expression of the chicken INCENPs in mammalian cells confirms that the signals and structures required for the transfer of these proteins from chromosomes to cytoskeleton are evolutionarily conserved. Furthermore, these studies reveal that INCENP association with the cytoskeleton is complex. The amino-terminal 42-amino acid residues are required for transfer of the INCENPs from the chromosomes to the mitotic spindle at anaphase, but not for binding of INCENPs to cytoplasmic microtubules. In contrast, an internal 200 amino acid coiled-coil domain was required for association with microtubules, but dispensable for spindle association. These experiments suggest that proteins required for assembly of specialized cytoskeletal structures during mitosis from anaphase onwards might be sequestered in the nucleus throughout interphase to keep them from disrupting the interphase cytoskeleton, and to ensure their correct positioning during mitosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.