ArticleThe Journal of cell biology1993
Contractile activity and cell-cell contact regulate myofibrillar organization in cultured cardiac myocytes.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 87 citations in OpenAlex.
- Developmental and Evolutionary Heart Adaptations Through Structure-Function Relationships.Journal of cardiovascular development and disease · 2025Review
- Cell Architecture and Dynamics of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs) on Hydrogels with Spatially Patterned Laminin and N-Cadherin.ACS applied materials & interfaces · 2025Article
- Matrix Architecture and Mechanics Regulate Myofibril Organization, Costamere Assembly, and Contractility in Engineered Myocardial Microtissues.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Coupling to substrate adhesions drives the maturation of muscle stress fibers into myofibrils within cardiomyocytes.Molecular biology of the cell · 2020Article
- Article
- Engineered Microenvironments for Maturation of Stem Cell Derived Cardiac Myocytes.Theranostics · 2018Review
- Disassembly of myofibrils and potential imbalanced forces on Z-discs in cultured adult cardiomyocytes.Cytoskeleton (Hoboken, N.J.) · 2016Article
- The constant beat: cardiomyocytes adapt their forces by equal contraction upon environmental stiffening.Biology open · 2013Article
- The contribution of cellular mechanotransduction to cardiomyocyte form and function.Biomechanics and modeling in mechanobiology · 2012Article
- Focal adhesion kinase regulation of mechanotransduction and its impact on endothelial cell functions.Microvascular research · 2012Review
- Mechanotransduction: the role of mechanical stress, myocyte shape, and cytoskeletal architecture on cardiac function.Pflugers Archiv : European journal of physiology · 2011Review
- Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.PloS one · 2010Article
- The initial steps of myofibril assembly: integrins pave the way.Nature reviews. Molecular cell biology · 2009Review
- Toward physiological conditions for cell analyses: forces of heart muscle cells suspended between elastic micropillars.Biophysical journal · 2008Article
- Focal adhesion kinase and p130Cas mediate both sarcomeric organization and activation of genes associated with cardiac myocyte hypertrophy.Molecular biology of the cell · 2001Article
- Mechanisms of thin filament assembly in embryonic chick cardiac myocytes: tropomodulin requires tropomyosin for assembly.The Journal of cell biology · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Adult feline ventricular myocytes cultured on a laminin-coated substratum reestablish intercellular junctions, yet disassemble their myofibrils. Immunofluorescence microscopy reveals that these non-beating heart cells lack vinculin-positive focal adhesions; moreover, intercellular junctions are also devoid of vinculin. When these quiescent myocytes are stimulated to contract with the beta-adrenergic agonist, isoproterenol, extensive vinculin-positive focal adhesions and intercellular junctions emerge. If solitary myocytes are stimulated to beat, an elaborate series of vinculin-positive focal adhesions develop which appear to parallel the reassembly of myofibrils. In cultures where neighboring myocytes reestablish cell-cell contact, myofibrils appear to reassemble from the fascia adherens rather than focal contacts. Activation of beating is accompanied by a significant reduction in the rate of total and cytoskeletal protein synthesis; in fact, myofibrillar reassembly, redevelopment of focal adhesions and fascia adherens junctions require no protein synthesis for at least 24 h, implying the existence of an assembly competent pool of cytoskeletal proteins. Maturation of the fasciae adherens and the appearance of vinculin within Z-line/costameres, does require de novo synthesis of new cytoskeletal proteins. Changes in cytoskeletal protein turnover appear dependent on beta agonist-induced cAMP production, but myofibrillar reassembly is a cAMP-independent event. Such observations suggest that mechanical forces, in the guise of contractile activity, regulate vinculin distribution and myofibrillar order in cultured adult feline heart cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.