ArticleThe Journal of cell biology1993
The actin-binding protein comitin (p24) is a component of the Golgi apparatus.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 105 citations in OpenAlex.
- Force balances between interphase centrosomes as revealed by laser ablation.Molecular biology of the cell · 2019Article
- A tripeptidyl peptidase 1 is a binding partner of the Golgi pH regulator (GPHR) inDisease models & mechanisms · 2017Article
- The centrosomal component CEP161 of Dictyostelium discoideum interacts with the Hippo signaling pathway.Cell cycle (Georgetown, Tex.) · 2015Article
- The Dictyostelium discoideum GPHR ortholog is an endoplasmic reticulum and Golgi protein with roles during development.Eukaryotic cell · 2015Article
- Actin acting at the Golgi.Histochemistry and cell biology · 2013Review
- There is more than one way to model an elephant. Experiment-driven modeling of the actin cytoskeleton.Biophysical journal · 2013Review
- CP55, a novel key component of centrosomal organization in Dictyostelium.Cellular and molecular life sciences : CMLS · 2012Article
- Article
- Seven kinds of intermediate filament networks in the cytoplasm of polarized cells: structure and function.Acta histochemica et cytochemica · 2010Article
- A cytohesin homolog in Dictyostelium amoebae.PloS one · 2010Article
- Yersinia outer protein YopE affects the actin cytoskeleton in Dictyostelium discoideum through targeting of multiple Rho family GTPases.BMC microbiology · 2009Article
- Regulation of the formation and trafficking of vesicles from Golgi by PCH family proteins during chemotaxis.Biochimica et biophysica acta · 2009Article
- Role of SP65 in assembly of the Dictyostelium discoideum spore coat.Eukaryotic cell · 2007Article
- GxcDD, a putative RacGEF, is involved in Dictyostelium development.BMC cell biology · 2007Article
- Dictyostelium LIS1 is a centrosomal protein required for microtubule/cell cortex interactions, nucleus/centrosome linkage, and actin dynamics.Molecular biology of the cell · 2005Article
- The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules.BMC cell biology · 2004Article
- Villidin, a novel WD-repeat and villin-related protein from Dictyostelium, is associated with membranes and the cytoskeleton.Molecular biology of the cell · 2003Article
- The AP-1 clathrin-adaptor is required for lysosomal enzymes sorting and biogenesis of the contractile vacuole complex in Dictyostelium cells.Molecular biology of the cell · 2003Article
- Loss of the F-actin binding and vesicle-associated protein comitin leads to a phagocytosis defect.Eukaryotic cell · 2002Article
- A myosin I is involved in membrane recycling from early endosomes.The Journal of cell biology · 2000Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comitin (p24) was first identified in Dictyostelium discoideum as a membrane-associated protein which binds in gel overlay assays to G and F actin. To analyze its actin-binding properties we used purified, bacterially expressed comitin and found that it binds to F actin in spin down experiments and increases the viscosity of F actin solutions even under high-salt conditions. Immunofluorescence studies, cell fractionation experiments and EM studies of vesicles precipitated with comitin-specific monoclonal antibodies showed that comitin was present in D. discoideum on: (a) a perinuclear structure with tubular or fibrillary extensions; and (b) on vesicles distributed throughout the cell. In immunofluorescence experiments using comitin antibodies NIH 3T3 fibroblasts showed a similar staining pattern as D. discoideum cells. Using bona fide Golgi markers the perinuclear structure was identified as the Golgi apparatus. The results were supported by an electron microscopic study using cryosections. Based on these data we propose that also in Dictyostelium the stained perinuclear structure is the Golgi apparatus. In vivo the perinuclear structure was found to be attached to the actin and the microtubule network. Alteration of the actin network or depolymerization of the microtubules led to its dispersal into vesicles distributed throughout the cell. These results suggest that the Golgi apparatus in D. discoideum is connected to the actin network by comitin. This protein seems also to be present in mammalian cells.
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