ArticleThe Journal of cell biology1993
Localization and dynamics of nonfilamentous actin in cultured cells.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed.
- DNase I as a probe for unpolymerized actin: revisiting a classic tool for nuclear actin research.Pflugers Archiv : European journal of physiology · 2026Review
- The effects of 808-nm near-infrared laser light irradiation on actin cytoskeleton reorganization in bone marrow mesenchymal stem cells.Cell and tissue research · 2021Article
- Thymosin β4 is essential for adherens junction stability and epidermal planar cell polarity.Development (Cambridge, England) · 2020Article
- Reconsidering an active role for G-actin in cytoskeletal regulation.Journal of cell science · 2018Review
- Recent advances in cytokinesis: understanding the molecular underpinnings.F1000Research · 2018Review
- Quantifying cadherin mechanotransduction machinery assembly/disassembly dynamics using fluorescence covariance analysis.Scientific reports · 2016Article
- Comparison of [corrected] actin- and glass-supported phospholipid bilayer diffusion coefficients.Biophysical journal · 2015Article
- The β-actin mRNA zipcode regulates epithelial adherens junction assembly but not maintenance.RNA (New York, N.Y.) · 2014Article
- Computational prediction of actin-actin interaction.Molecular biology reports · 2014Article
- Dynamic localization of G-actin during membrane protrusion in neuronal motility.Current biology : CB · 2013Article
- The interaction between actin and FA fragment of diphtheria toxin.Molecular biology reports · 2013Article
- Low-dose etoposide-treatment induces endoreplication and cell death accompanied by cytoskeletal alterations in A549 cells: Does the response involve senescence? The possible role of vimentin.Cancer cell international · 2013Article
- Myo1c facilitates G-actin transport to the leading edge of migrating endothelial cells.The Journal of cell biology · 2012Article
- α-Actinin-4/FSGS1 is required for Arp2/3-dependent actin assembly at the adherens junction.The Journal of cell biology · 2012Article
- Features of senescence and cell death induced by doxorubicin in A549 cells: organization and level of selected cytoskeletal proteins.Journal of cancer research and clinical oncology · 2010Article
- Regulation of acetylcholine receptor clustering by ADF/cofilin-directed vesicular trafficking.Nature neuroscience · 2009Article
- Spatial coordination of actin polymerization and ILK-Akt2 activity during endothelial cell migration.Developmental cell · 2009Article
- Visualization of mRNA translation in living cells.The Journal of cell biology · 2006Article
- Distribution of G-actin is related to root hair growth of wheat.Annals of botany · 2006Article
- Localization of selenium-binding protein at the tips of rapidly extending protrusions.Histochemistry and cell biology · 2004Article
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
Although the distribution of filamentous actin is well characterized in many cell types, the distribution of nonfilamentous actin remains poorly understood. To determine the relative distribution of filamentous and nonfilamentous actin in cultured NRK cells, we have used a number of labeling agents that differ with respect to their specificities toward the filamentous or nonfilamentous form, including monoclonal and polyclonal anti-actin antibodies, vitamin D-binding protein (DBP), and fluorescent phalloidin. Numerous punctate structures were identified that bind poorly to phalloidin but stain positively with several anti-actin antibodies. These bead structures also stain with DBP, suggesting that they are enriched in nonfilamentous actin. Similar punctate structures were observed after the microinjection of fluorescently labeled actin into living cells, allowing us to examine their dynamics in living cells. The actin-containing punctate structures were observed predominantly in the region behind lamellipodia, particularly in spreading cells induced by wounding confluent monolayers. Time-lapse recording of cells injected with fluorescent actin indicated that they form continuously near the leading edge and move centripetally toward the nucleus. Our results suggest that at least part of the unpolymerized actin molecules are localized at discrete sites, possibly as complexes with monomer sequestering proteins. These structures may represent transient storage sites of G-actin within the cell which can be transformed rapidly into actin filaments upon stimulation by specific signals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.