ArticleJournal of bacteriology1993
Isolation and analysis of eight exe genes and their involvement in extracellular protein secretion and outer membrane assembly in Aeromonas hydrophila.
Article in Journal of bacteriology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 86 citations in OpenAlex.
- Article
- Suppressor Mutations in Type II Secretion Mutants of Vibrio cholerae: Inactivation of the VesC Protease.mSphere · 2020Article
- Expanding Role of Type II Secretion in Bacterial Pathogenesis and Beyond.Infection and immunity · 2017Review
- A Putative Type II Secretion System Is Involved in Cellulose Utilization inFrontiers in microbiology · 2017Article
- The type II secretion system is essential for erythrocyte lysis and gut colonization by the leech digestive tract symbiont Aeromonas veronii.Applied and environmental microbiology · 2011Article
- Cell envelope perturbation induces oxidative stress and changes in iron homeostasis in Vibrio cholerae.Journal of bacteriology · 2009Article
- Compromised outer membrane integrity in Vibrio cholerae Type II secretion mutants.Journal of bacteriology · 2007Article
- Purification and characterization of the N-terminal domain of ExeA: a novel ATPase involved in the type II secretion pathway of Aeromonas hydrophila.Journal of bacteriology · 2005Article
- Analysis of the pilU gene for the prepilin peptidase involved in the biogenesis of type IV pili encoded by plasmid R64.Molecular genetics and genomics : MGG · 2005Article
- Legionella pneumophila type II protein secretion promotes virulence in the A/J mouse model of Legionnaires' disease pneumonia.Infection and immunity · 2004Article
- Identification of XcpZ domains required for assembly of the secreton of Pseudomonas aeruginosa.Journal of bacteriology · 2002Article
- Type II secretion and pathogenesis.Infection and immunity · 2001Review
- Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion.The EMBO journal · 2000Article
- Investigation of the role of type IV Aeromonas pilus (Tap) in the pathogenesis of Aeromonas gastrointestinal infection.Infection and immunity · 2000Article
- Influence of deletions within domain II of exotoxin A on its extracellular secretion from Pseudomonas aeruginosa.Journal of bacteriology · 2000Article
- Association of the cytoplasmic membrane protein XpsN with the outer membrane protein XpsD in the type II protein secretion apparatus of Xanthomonas campestris pv. campestris.Journal of bacteriology · 2000Article
- Two regions of EpsL involved in species-specific protein-protein interactions with EpsE and EpsM of the general secretion pathway in Vibrio cholerae.Journal of bacteriology · 2000Article
- Legionella pneumophila contains a type II general secretion pathway required for growth in amoebae as well as for secretion of the Msp protease.Infection and immunity · 1999Article
- Direct interaction of the EpsL and EpsM proteins of the general secretion apparatus in Vibrio cholerae.Journal of bacteriology · 1999Article
- Genetic and transcriptional analyses of the Vibrio cholerae mannose-sensitive hemagglutinin type 4 pilus gene locus.Journal of bacteriology · 1999Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The exeE gene of Aeromonas hydrophila has been shown to be required for the secretion of most if not all of the extracellular proteins produced by this bacterium. In addition, an exeE::Tn5-751 insertion mutant of A. hydrophila was found to be deficient in the amounts of a number of its major outer membrane proteins (B. Jiang and S. P. Howard, J. Bacteriol. 173:1241-1249, 1991). The exeE gene and the exeF gene were previously isolated as part of a fragment which complemented this mutant. In this study, we have isolated and sequenced a further eight exe genes, exeG through exeN, which constitute the 3' end of the exe operon. These genes have a high degree of similarity with the extracellular secretion operons of a number of different gram-negative bacteria. Marker exchange mutagenesis was used to insert kanamycin resistance cassettes into three different regions of the exe operon. The phenotypes of these mutants showed that in A. hydrophila this operon is required not only for extracellular protein secretion but also for normal assembly of the outer membrane, in particular with respect to the quantities of the major porins. Five of the Exe proteins contain type IV prepilin signal sequences, although the prepilin peptidase gene does not appear to form part of the exe operon. Limited processing of the ExeG protein was observed when it was expressed in Escherichia coli, and this processing was greatly accelerated in the presence of the prepilin peptidase of Pseudomonas aeruginosa.
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