ArticleInfection and immunity1993
Modulation of adjuvant arthritis in Lewis rats by recombinant vaccinia virus expressing the human 60-kilodalton heat shock protein.
Article in Infection and immunity, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 39 citations in OpenAlex.
- HSP: Bystander Antigen in Atopic Diseases?Frontiers in immunology · 2012Article
- Heat-shock proteins can promote as well as regulate autoimmunity.Autoimmunity reviews · 2009Review
- Regulation of autoimmune arthritis by self-heat-shock proteins.Trends in immunology · 2008Article
- HSP60 as a target of anti-ergotypic regulatory T cells.PloS one · 2008Article
- Heat-shock proteins induce T-cell regulation of chronic inflammation.Nature reviews. Immunology · 2005Review
- DNA vaccination with CD25 protects rats from adjuvant arthritis and induces an antiergotypic response.The Journal of clinical investigation · 2004Article
- Expression of normal and truncated forms of human endoglin.The Biochemical journal · 1999Article
- Hsp70: a carrier molecule with built-in adjuvanticity.Experientia · 1994Review
- Self heat-shock protein 65-mediated regulation of autoimmune arthritis.Journal of autoimmunityArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The immune response to the mycobacterial 65-kDa heat shock protein (hsp65) is considered an important event in the induction of adjuvant arthritis (AA) in rats; this induction probably occurs through a molecular mimicry mechanism involving cross-reactivity against the rat homolog hsp60. To analyze the role of mammalian molecule hsp60 in arthritis, we generated a recombinant vaccinia virus (hsp60-VV) carrying the human hsp60 gene inserted into the thymidine kinase locus under the control of the 7.5k vaccinia virus promoter. Human hsp60 is almost identical to its rat homolog (97.4% linear amino acid homology) and shares about 50% of amino acid positions with Mycobacterium tuberculosis hsp65. The latter supposedly carries a critical epitope for AA induction that is not present in human hsp60. Infections with hsp60-VV of monkey cell cultures led to the expression of the human hsp60 molecule, as evidenced by immunoblotting analysis with specific monoclonal antibodies. Also, Lewis rats infected with hsp60-VV produced specific antibodies, demonstrating the in vivo expression of human hsp60 in the infected animals. Therefore, we used hsp60-VV to analyze whether the delivery of hsp60 could affect the induction of AA in Lewis rats. hsp60-VV clearly reduced and retarded arthritic symptoms when administered to rats at day 7 after AA induction. In contrast, inoculation of rats with a control recombinant vaccinia virus did not affect the course of the disease. The improvement in AA with hsp60-VV administration was associated with a specific immune response, as determined by the presence of antibodies to hsp60 in the sera and the proliferation induced by hsp60 of T cells from popliteal lymph nodes. These results support a critical role for immunity to heat shock proteins in AA. Since the protective construct is virtually identical to rat homolog hsp60, we conclude that immunity directed to conserved areas of this family of proteins is directly involved in the pathogenesis of AA.
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