ArticleThe EMBO journal1993
Phosphorylation of KSP motifs in the C-terminal region of titin in differentiating myoblasts.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 113 citations in OpenAlex.
- RHOT Proteins Link Mitochondrial Motility to Cardiomyocyte Sarcomere Maturation.Circulation research · 2026Article
- Temporal involvement of phosphatidylinositol 4-phosphate 5-kinase γ in differentiation of Z-bands and myofilament bundles as well as intercalated discs in mouse heart at mid-gestation.Journal of anatomy · 2024Article
- Alterations in cytoskeletal and CaFrontiers in cardiovascular medicine · 2023Article
- Titin (TTN): from molecule to modifications, mechanics, and medical significance.Cardiovascular research · 2022Review
- Proteomic and phosphoproteomic profiling in heart failure with preserved ejection fraction (HFpEF).Frontiers in cardiovascular medicine · 2022Article
- Making sense of missense variants in TTN-related congenital myopathies.Acta neuropathologica · 2021Article
- Structural diversity in the atomic resolution 3D fingerprint of the titin M-band segment.PloS one · 2019Article
- Thick Filament Protein Network, Functions, and Disease Association.Comprehensive Physiology · 2018Review
- When signalling goes wrong: pathogenic variants in structural and signalling proteins causing cardiomyopathies.Journal of muscle research and cell motility · 2017Review
- Tampering with springs: phosphorylation of titin affecting the mechanical function of cardiomyocytes.Biophysical reviews · 2017Review
- The sarcomeric M-region: a molecular command center for diverse cellular processes.BioMed research international · 2015Review
- Tuning the molecular giant titin through phosphorylation: role in health and disease.Trends in cardiovascular medicine · 2013Review
- Conformation-regulated mechanosensory control via titin domains in cardiac muscle.Pflugers Archiv : European journal of physiology · 2011Review
- The giant protein titin: a regulatory node that integrates myocyte signaling pathways.The Journal of biological chemistry · 2011Review
- Titin-based mechanosensing and signaling: role in diaphragm atrophy during unloading?American journal of physiology. Lung cellular and molecular physiology · 2011Review
- Bin1 SRC homology 3 domain acts as a scaffold for myofiber sarcomere assembly.The Journal of biological chemistry · 2009Article
- Muscle giants: molecular scaffolds in sarcomerogenesis.Physiological reviews · 2009Review
- Studies on titin PEVK peptides and their interaction.Archives of biochemistry and biophysics · 2006Article
- Protein kinase-A phosphorylates titin in human heart muscle and reduces myofibrillar passive tension.Journal of muscle research and cell motility · 2006Article
- Titin: physiological function and role in cardiomyopathy and failure.Heart failure reviews · 2005Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Titin is a giant structural protein of striated muscle (M(r) approximately 3000 kDa) and single molecules span sarcomeres from the M- to Z-lines. We have cloned and sequenced the C-terminal region of the titin molecule, which is an integral part of M-lines and forms intimate contacts with the 165 and 190 kDa M-line proteins. In contrast to the regular motif patterns of the A-band portion of titin, the 5.7 kb of titin sequences from the M-line show a complex structure of immunoglobulin-C2 repeats, separated by unique interdomain insertion sequences. As a striking feature, one interdomain insertion comprises four KSP repeats analogous to the multi-phosphorylation repeats of neurofilament subunits H and M. In vitro phosphorylation assays with expressed titin KSP sequences detect high levels of titin KSP phosphorylating kinases in developing but not in differentiated muscle. Since this kinase activity can be depleted from myocyte extracts by antibodies against cdc2 kinase and p13suc1 beads, the titin KSP kinase is structurally related to cdc2 kinase. We suggest that titin C-terminal phosphorylation by SP-specific kinases is regulated during differentiation, and that this may control the assembly of M-line proteins into regular structures during myogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.