ArticleThe EMBO journal1993
The metaphase II arrest in mouse oocytes is controlled through microtubule-dependent destruction of cyclin B in the presence of CSF.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 273 citations in OpenAlex.
- MOS is a key regulator of meiotic midbody assembly and prevents abnormal divisions in mouse eggs and their polar bodies.Journal of cell science · 2026Article
- Induction of experimental cell division to generate cells with reduced chromosome ploidy.Nature communications · 2025Article
- No transcription, no problem: Protein phosphorylation changes and the transition from oocyte to embryo.Current topics in developmental biology · 2025Review
- Oocyte and embryo culture under oil profoundly alters effective concentrations of small molecule inhibitors.Frontiers in cell and developmental biology · 2024Article
- Meiotic Cell Cycle Progression in Mouse Oocytes: Role of Cyclins.International journal of molecular sciences · 2023Review
- Recent advances in mammalian reproductive biology.Science China. Life sciences · 2020Review
- Positive Feedback Stimulation ofFrontiers in cell and developmental biology · 2020Article
- Cycling through mammalian meiosis: B-type cyclins in oocytes.Cell cycle (Georgetown, Tex.) · 2019Article
- Reduction of phosphorylated Thr-161 Cdk1 level participates in roscovitine-induced Fas ligand-mediated apoptosis in rat eggs cultured in vitro.In vitro cellular & developmental biology. Animal · 2015Article
- Effect of demecolcine-assisted enucleation on the MPF level and cyclin B1 distribution in porcine oocytes.PloS one · 2014Article
- Article
- Modulation of cell cycle control during oocyte-to-embryo transitions.The EMBO journal · 2013Review
- Hec1-dependent cyclin B2 stabilization regulates the G2-M transition and early prometaphase in mouse oocytes.Developmental cell · 2013Article
- Cdc25A activity is required for the metaphase II arrest in mouse oocytes.Journal of cell science · 2013Article
- A zinc-dependent mechanism regulates meiotic progression in mammalian oocytes.Biology of reproduction · 2012Article
- Essential role of protein phosphatase 2A in metaphase II arrest and activation of mouse eggs shown by okadaic acid, dominant negative protein phosphatase 2A, and FTY720.The Journal of biological chemistry · 2011Article
- Article
- Gas6 downregulation impaired cytoplasmic maturation and pronuclear formation independent to the MPF activity.PloS one · 2011Article
- Second meiotic arrest and exit in frogs and mice.EMBO reports · 2008Review
- Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta.BMC developmental biology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In unfertilized eggs from vertebrates, the cell cycle is arrested in metaphase of the second meiotic division (metaphase II) until fertilization or activation. Maintenance of the long-term meiotic metaphase arrest requires mechanisms preventing the destruction of the maturation promoting factor (MPF) and the migration of the chromosomes. In frog oocytes, arrest in metaphase II (M II) is achieved by cytostatic factor (CSF) that stabilizes MPF, a heterodimer formed of cdc2 kinase and cyclin. At the metaphase/anaphase transition, a rapid proteolysis of cyclin is associated with MPF inactivation. In Drosophila, oocytes are arrested in metaphase I (M I); however, only mechanical forces generated by the chiasmata seem to prevent chromosome separation. Thus, entirely different mechanisms may be involved in the meiotic arrests in various species. We report here that in mouse oocytes a CSF-like activity is involved in the M II arrest (as observed in hybrids composed of fragments of metaphase II-arrested oocytes and activated mitotic mouse oocytes) and that the high activity of MPF is maintained through a continuous equilibrium between cyclin B synthesis and degradation. In addition, the presence of an intact metaphase spindle is required for cyclin B degradation. Finally, MPF activity is preferentially associated with the spindle after bisection of the oocyte. Taken together, these observations suggest that the mechanism maintaining the metaphase arrest in mouse oocytes involves an equilibrium between cyclin synthesis and degradation, probably controlled by CSF, and which is also dependent upon the three-dimensional organization of the spindle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.