ArticleThe EMBO journal1993
Identification of Exo2 as the catalytic subunit of protein kinase A reveals a role for cyclic AMP in Ca(2+)-dependent exocytosis in chromaffin cells.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- Protein kinase B/Akt is a novel cysteine string protein kinase that regulates exocytosis release kinetics and quantal size.The Journal of biological chemistry · 2006Article
- Cyclic AMP induces integrin-mediated cell adhesion through Epac and Rap1 upon stimulation of the beta 2-adrenergic receptor.The Journal of cell biology · 2003Article
- Long-term potentiation of exocytosis and cell membrane repair in fibroblasts.Molecular biology of the cell · 2003Article
- Lysosomes and the plasma membrane: trypanosomes reveal a secret relationship.The Journal of cell biology · 2002Review
- Protein kinase C-dependent and Ca2+-dependent mechanisms of secretion from streptolysin O-permeabilized platelets: effects of leakage of cytosolic proteins.The Biochemical journal · 1997Article
- Modification of annexin II expression in PC12 cell lines does not affect Ca(2+)-dependent exocytosis.Molecular biology of the cell · 1997Article
- Phosphorylation of synaptic vesicle proteins: modulation of the alpha SNAP interaction with the core complex.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Purification and identification of FOAD-II, a cytosolic protein that regulates secretion in streptolysin-O permeabilized mast cells, as a rac/rhoGDI complex.Molecular biology of the cell · 1996Article
- Soluble N-ethylmaleimide-sensitive-factor attachment protein and N-ethylmaleimide-insensitive factors are required for Ca2+-stimulated exocytosis of insulin.The Biochemical journal · 1996Article
- Distinct effects of alpha-SNAP, 14-3-3 proteins, and calmodulin on priming and triggering of regulated exocytosis.The Journal of cell biology · 1995Article
- Molecular mechanisms in exocytosis.The Journal of membrane biology · 1995Review
- Fast exocytosis and endocytosis triggered by depolarisation in single adrenal chromaffin cells before rapid Ca2+ current run-down.Pflugers Archiv : European journal of physiology · 1995Article
- A role for soluble NSF attachment proteins (SNAPs) in regulated exocytosis in adrenal chromaffin cells.The EMBO journal · 1995Article
- A reassessment of the modulatory role of cyclic AMP in catecholamine secretion by chromaffin cells.British journal of pharmacology · 1995Article
- Characterization of 14-3-3 proteins in adrenal chromaffin cells and demonstration of isoform-specific phospholipid binding.The Biochemical journal · 1994Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Digitonin-permeabilized chromaffin cells secrete catecholamines by exocytosis in response to micromolar Ca2+ concentrations, but lose the ability to secrete in response to Ca2+ as the cells lose soluble proteins through the plasma membrane pores. We have previously shown [Morgan and Burgoyne (1992) Nature, 355, 833-836] that cytosol can retard this loss of secretory competence and that two distinct stimulatory activities (Exo1 and Exo2) are present in cytosol. Here we report that Exo2 behaved as a single peak of activity through purification on hydroxyapatite, ammonium sulfate precipitation and gel filtration and the activity correlated with a single polypeptide of approximately 44 kDa on SDS gels. Protein sequencing of this band revealed it to be the catalytic subunit of cyclic AMP-dependent protein kinase (PKA). Both cyclic AMP and the commercially available catalytic subunit of PKA stimulated exocytosis in a dose-dependent manner which was absolutely dependent on the presence of micromolar Ca2+. These data show that PKA (Exo2) regulates Ca(2+)-dependent exocytosis in bovine adrenal chromaffin cells.
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