ArticlePlant molecular biology1993
Structure and expression of a nuclear gene for the PSI-D subunit of photosystem I in Nicotiana sylvestris.
Article in Plant molecular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 26 citations in OpenAlex.
- Composition and structure of photosystem I in the moss Physcomitrella patens.Journal of experimental botany · 2013Article
- Genome-wide computational prediction and analysis of core promoter elements across plant monocots and dicots.PloS one · 2013Article
- Characteristics of core promoter types with respect to gene structure and expression in Arabidopsis thaliana.DNA research : an international journal for rapid publication of reports on genes and genomes · 2011Article
- Changes induced by the Pepper mild mottle tobamovirus on the chloroplast proteome of Nicotiana benthamiana.Photosynthesis research · 2010Article
- Nucleotide sequence of the psaD gene from the thermophilic cyanobacterium Synechococcus vulcanus.Photosynthesis research · 1995Article
- Cloning of a nuclear-encoded photosystem I gene, psaEb, in Nicotiana sylvestris.Plant physiology · 1995Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The PSI-D subunit is the ferredoxin-binding site of photosystem I, and is encoded by the nuclear gene psaD. We isolated a psaD genomic clone from Nicotiana sylvestris, by screening a genomic library with a psaD cDNA which we previously cloned from N. sylvestris (Yamamoto et al., Plant Mol Biol 17: 1251, 1991). Nucleotide sequence analysis revealed that this genomic clone contains a psaD gene, which does not correspond to the psaD cDNA, so we designated these genes psaDb and psaDa, respectively. The psaDb clone encodes a protein of 214 amino acids uninterrupted by introns. The N-terminal sequence determined for the N. sylvestris PSI-D protein encoded by psaDb begins at the 49th residue. The products of psaDa and psaDb share 82.7% and 79.5% identity at the amino acid and nucleotide levels, respectively. Genomic Southern analysis showed that two copies of psaD are present in the N. sylvestris genome. Ribonuclease protection assays and immunoblot analysis in N. sylvestris indicate that both genes are expressed in leaves, stems and flower buds, but neither is expressed in roots. During leaf development, the ratio of psaDb to psaDa mRNA increases from 0.12 in leaf buds to 0.36 in mature leaves. The relative abundance of the corresponding proteins decreased over the same developmental period. These results indicate that differential regulation mechanisms control psaDa and psaDb expression at both the mRNA and protein levels during leaf development.
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