ArticlePlant molecular biology1993
Epigenetic repeat-induced gene silencing (RIGS) in Arabidopsis.
Article in Plant molecular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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Who cites it
91 citing papers in PubMed.
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- Turbo-charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond.The Plant journal : for cell and molecular biology · 2025Review
- A compendium of nonredundant short polymerase III promoters for CRISPR applications.Plant physiology · 2025Article
- Harnessing haploid-inducer mediated genome editing for accelerated maize variety development.Plant biotechnology journal · 2025Article
- Computationally derived RNA polymerase III promoters enable maize genome editing.Frontiers in plant science · 2025Article
- Tissue-specific silencing of integrated transgenes achieved through endogenous RNA interference inRNA biology · 2024Article
- Intron-mediated enhancement of DIACYLGLYCEROL ACYLTRANSFERASE1 expression in energycane promotes a step change for lipid accumulation in vegetative tissues.Biotechnology for biofuels and bioproducts · 2023Article
- Transgene Was Silenced in Hybrids between Transgenic Herbicide-Resistant Crops and Their Wild Relatives Utilizing Alien Chromosomes.Plants (Basel, Switzerland) · 2022Article
- Increased rates of gene-editing events using a simplified RNAi configuration designed to reduce gene silencing.Plant cell reports · 2022Article
- A single promoter-TALE system for tissue-specific and tuneable expression of multiple genes in rice.Plant biotechnology journal · 2022Article
- Optimization of T-DNA configuration with UBIQUITIN10 promoters and tRNA-sgRNA complexes promotes highly efficient genome editing in allotetraploid tobacco.Plant cell reports · 2022Article
- Rapid Macrosatellite Evolution Promotes X-Linked Hybrid Male Sterility in a Feline Interspecies Cross.Molecular biology and evolution · 2021Article
- Ectopic Expression of a Maize Gene Is Induced by Composite Insertions Generated Through Alternative Transposition.Genetics · 2020Article
- RNA-directed DNA Methylation.PLoS genetics · 2020Article
- A copy number variant is associated with a spectrum of pigmentation patterns in the rock pigeon (Columba livia).PLoS genetics · 2020Article
- High-Level Production of Recombinant Snowdrop Lectin in Sugarcane and Energy Cane.Frontiers in bioengineering and biotechnology · 2020Article
- Transformation strategies for stable expression of complex hetero-multimeric proteins like secretory immunoglobulin A in plants.Plant biotechnology journal · 2019Article
- Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane.Plant molecular biology · 2019Article
- Partition of Repeat-Induced Point Mutations Reveals Structural Aspects of Homologous DNA-DNA Pairing.Biophysical journal · 2018Article
- Emerging roles of macrosatellite repeats in genome organization and disease development.Epigenetics · 2017Review
31 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
In several plant systems expression of structurally intact genes may be silenced epigenetically when a transgenic construct increases the copy number of DNA sequences. Here we report epigenetic silencing in Arabidopsis lines containing transgenic inserts of defined genetic structure, all at the same genomic locus. These comprise an allelic series that includes a single copy of the primary insert, which carries repeated drug resistance transgenes, and a set of its derivatives, which as a result of recombination within the insert carry different numbers and alleles of resistance genes. Although the drug resistance genes remained intact, both the primary and some recombinant lines nevertheless segregated many progeny that were partly or fully drug-sensitive because of silencing. As in other systems silencing was reversible, and correlated with decreased steady-state mRNA and increased DNA methylation. Each different number and combination of genes, on the same or different (i.e., homologous) chromosomes, conditioned its own idiosyncratic segregation pattern. Strikingly, lines with a single gene segregated only a few slightly drug-sensitive progeny whereas multi-gene lines segregated many highly sensitive progeny, indicating dependence of silencing at this locus on repeated sequences. This argues strongly against explanations based on antisense RNA, but is consistent with explanations based on ectopic DNA pairing. One possibility is that silencing reflects the interaction of paired homologous DNA with flanking heterologous DNA, which induces condensation of chromatin into a non-transcribable state.
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Registered trials
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