ArticlePflugers Archiv : European journal of physiology1993
Fast Na+ current in circular smooth muscle cells of the large intestine.
Article in Pflugers Archiv : European journal of physiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 42 citations in OpenAlex.
- The role of mechanosensitive ion channels in the gastrointestinal tract.Frontiers in physiology · 2022Review
- Irritable bowel syndrome patients have SCN5A channelopathies that lead to decreased NaAmerican journal of physiology. Gastrointestinal and liver physiology · 2018Article
- Modelling Human Colonic Smooth Muscle Cell Electrophysiology.Cellular and molecular bioengineering · 2017Article
- Expression and function of the Scn5a-encoded voltage-gated sodium channel NaV 1.5 in the rat jejunum.Neurogastroenterology and motility · 2016Article
- Ranolazine inhibits voltage-gated mechanosensitive sodium channels in human colon circular smooth muscle cells.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Loss-of-function of the voltage-gated sodium channel NaV1.5 (channelopathies) in patients with irritable bowel syndrome.Gastroenterology · 2014Article
- Targeting ion channels for the treatment of gastrointestinal motility disorders.Therapeutic advances in gastroenterology · 2012Article
- Hydrogen sulfide is a partially redox-independent activator of the human jejunum Na+ channel, Nav1.5.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- Identification of functional voltage-gated Na(+) channels in cultured human pulmonary artery smooth muscle cells.Pflugers Archiv : European journal of physiology · 2005Article
- Novel voltage-dependent non-selective cation conductance in murine colonic myocytes.The Journal of physiology · 2001Article
- Membrane currents in cultured human intestinal smooth muscle cells.The Journal of physiology · 2000Article
- Expression and functional analysis of voltage-activated Na+ channels in human prostate cancer cell lines and their contribution to invasion in vitro.The American journal of pathology · 1997Article
- Potassium currents in rat colonic smooth muscle cells and changes during development and aging.Pflugers Archiv : European journal of physiology · 1995Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Whole-cell voltage clamp was carried out on freshly dispersed single smooth muscle cells from adult rat and human colons to investigate the regulation of the Ca2+ channels. In this study, we unexpectedly discovered the existence of a fast Na+ channel current. With normal physiological salt solution (PSS) plus 4-amino-pyridine (3 mM) in the bath and high-Cs+ solution in the pipette to inhibit outward K+ currents, an inward current possessing fast and slow components was observed when the cell membrane was depolarized to a value more positive than -20 mV from a holding potential of -100 mV. When Ca2+ ions were removed from the PSS, or when nifedipine (10 microM) and Ni2+ (30 microM) were simultaneously applied, the slow component disappeared and the fast component remained. The fast current component became almost completely inactivated within 10 ms. This fast component was dependent on extracellular Na+ concentration and was inhibited by tetrodotoxin (TTX) dose dependently (IC50 of 130 nM in rat and 14 nM in human). These results suggest that the slow component of inward current was a Ca2+ channel current, whereas the fast component was a TTX-sensitive fast Na+ channel current. The threshold voltage, the voltage for peak current, and the reversal potential for the fast Na+ current were, respectively, about -50, -20, and +50 mV in rats, and -40, 0, and +60 mV in humans. The incidence of cells possessing fast Na+ currents depended on the region of the colon.(ABSTRACT TRUNCATED AT 250 WORDS)
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.