ArticleGenetics1993
Isolation of the Chlamydomonas regulatory gene NIT2 by transposon tagging.
Article in Genetics, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 174 citations in OpenAlex.
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- Type II metacaspase mediates light-dependent programmed cell death in Chlamydomonas reinhardtii.Plant physiology · 2024Article
- Engineered production of isoprene from the model green microalgaMetabolic engineering communications · 2023Article
- The Chlamydomonas Genome Project, version 6: Reference assemblies for mating-type plus and minus strains reveal extensive structural mutation in the laboratory.The Plant cell · 2023Article
- Phosphoribulokinase abundance is not limiting the Calvin-Benson-Bassham cycle inFrontiers in plant science · 2023Article
- How abiotic stress-induced socialization leads to the formation of massive aggregates in Chlamydomonas.Plant physiology · 2022Article
- Nitrous Oxide Emissions from Nitrite Are Highly Dependent on Nitrate Reductase in the MicroalgaInternational journal of molecular sciences · 2022Article
- The Spermidine Synthase GeneCells · 2022Article
- Teaching an old 'doc' new tricks for algal biotechnology: Strategic filter use enables multi-scale fluorescent protein signal detection.Frontiers in bioengineering and biotechnology · 2022Article
- Combinatorial Engineering Enables Photoautotrophic Growth in High Cell Density Phosphite-Buffered Media to Support EngineeredFrontiers in microbiology · 2022Article
- Blasticidin S Deaminase: A New Efficient Selectable Marker forFrontiers in plant science · 2020Article
- FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia.Molecular biology of the cell · 2019Article
- Organelle Studies and Proteome Analyses of Mitochondria and Plastids Fractions from the Diatom Thalassiosira pseudonana.Plant & cell physiology · 2019Article
- Review
- Chlamydomonas Genome Resource for Laboratory Strains Reveals a Mosaic of Sequence Variation, Identifies True Strain Histories, and Enables Strain-Specific Studies.The Plant cell · 2015Article
- Carbon allocation and element composition in four Chlamydomonas mutants defective in genes related to the CO2 concentrating mechanism.Photosynthesis research · 2014Article
- High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA.The Plant cell · 2014Article
- Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism.The Plant cell · 2014Article
21 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic evidence suggests that the NIT2 gene of Chlamydomonas reinhardtii encodes a positive regulator of the nitrate-assimilation pathway. To learn more about the function of the NIT2 gene product, we isolated the gene using a transposon-tagging strategy. A nit2 mutation caused by the insertion of a transposon was identified by testing spontaneous nit2 mutants for the presence of new copies of Gulliver or TOC1, transposable elements that have been identified in Chlamydomonas. In 2 of the 14 different mutants that were analyzed, a Gulliver element was found to be genetically and phenotypically associated with the nit2 mutation. Using the Gulliver element as a probe, one of the transposon-induced nit2 alleles was isolated, and a sequence adjoining the transposon was used to isolate the corresponding wild-type locus. The NIT2 gene was delimited by mapping DNA rearrangements associated with nit2 mutations and mutant rescue by genetic transformation. The NIT2 gene encodes a 6-kb transcript that was not detected in cells grown in the presence of ammonium. Likewise, NIT2-dependent genes are repressed in ammonium-grown cells. These results suggest that repression of the NIT2 gene may mediate metabolite repression of the nitrate assimilation pathway in Chlamydomonas.
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