ArticleNucleic acids research1993
Biochemical and electron microscope analyses of the DNA reverse transcripts present in the virus-like particles of the yeast transposon Ty1. Identification of a second origin of Ty1DNA plus strand synthesis.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Host Factors Promoting the LTR Retrotransposon Life Cycle in Plant Cells: Current Knowledge and Future Directions.International journal of molecular sciences · 2025Review
- Becoming a Selfish Clan: Recombination Associated to Reverse-Transcription in LTR Retrotransposons.Genome biology and evolution · 2019Review
- Reverse Transcription in the Saccharomyces cerevisiae Long-Terminal Repeat Retrotransposon Ty3.Viruses · 2017Review
- The Ty1 LTR-retrotransposon of budding yeast,Microbiology spectrum · 2015Article
- Sgs1 RecQ helicase inhibits survival of Saccharomyces cerevisiae cells lacking telomerase and homologous recombination.The Journal of biological chemistry · 2008Article
- The take and give between retrotransposable elements and their hosts.Annual review of genetics · 2008Review
- Host factors that control long terminal repeat retrotransposons in Saccharomyces cerevisiae: implications for regulation of mammalian retroviruses.Eukaryotic cell · 2007Review
- Article
- A nucleocapsid functionality contained within the amino terminus of the Ty1 protease that is distinct and separable from proteolytic activity.Journal of virology · 2002Article
- An active retrotransposon in Candida albicans.Nucleic acids research · 2001Article
- A Ty1 reverse transcriptase active-site aspartate mutation blocks transposition but not polymerization.Journal of virology · 2001Article
- In vivo Ty1 reverse transcription can generate replication intermediates with untidy ends.Journal of virology · 1998Article
- Replication errors during in vivo Ty1 transposition are linked to heterogeneous RNase H cleavage sites.Molecular and cellular biology · 1998Article
- Plus-strand strong-stop DNA transfer in yeast Ty retrotransposons.The EMBO journal · 1997Article
- pCal, a highly unusual Ty1/copia retrotransposon from the pathogenic yeast Candida albicans.Journal of bacteriology · 1997Article
- Heterogeneous terminal structure of Ty1 and Ty3 reverse transcripts.Nucleic acids research · 1997Article
- Ty3 integrase mutants defective in reverse transcription or 3'-end processing of extrachromosomal Ty3 DNA.Journal of virology · 1996Article
- Plus-strand strong-stop DNA synthesis in retrotransposon Ty1.Journal of virology · 1995Article
- Yeast Ty1 retrotransposon: the minus-strand primer binding site and a cis-acting domain of the Ty1 RNA are both important for packaging of primer tRNA inside virus-like particles.Nucleic acids research · 1994Article
- The primer tRNA sequence is not inherited during Ty1 retrotransposition.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transposition of Saccharomyces cerevisiae Ty1 retroelements has been shown to involve reverse transcription in intracytoplasmic virus-like particles (Ty-VLPs). Ty DNA present in the particles specified by Ty1-H3 element was found to consist of the full-length genomic DNA as well as incomplete cDNAs mainly of plus polarity. Our results indicate that identical sequences (TGGGTGGTA) are used as primers for the synthesis of plus strand cDNA, generating cDNAs of 0.345 kb (analogous to the retroviral strong-stop plus cDNA) and of 2.1 kb. Electron microscopic analyses of Ty1-VLP DNA revealed two distinct classes, one full-length and the other corresponding to 0.34 kbp molecules, the size of a LTR sequence. The full-length molecules are either completely double-stranded or only partially double- stranded at one end or at both ends. These double-stranded regions are of a length corresponding to those of incomplete plus strands detected by biochemical techniques. Double-stranded circular molecules mainly of a length corresponding to that of two-LTR circles were also detected on electron micrographs. These analyses allowed us to propose a scheme for reverse transcription in Ty particles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.