ArticleProceedings of the National Academy of Sciences of the United States of America1993
Binding and transcriptional activation of the promoter for the neural cell adhesion molecule by HoxC6 (Hox-3.3).
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 101 citations in OpenAlex.
- Transcriptional Profiling Defines Unique Subtypes of Transit Amplifying Neural Progenitors Within the Neonatal Mouse Subventricular Zone.Biomolecules · 2025Article
- ACAM, a novel member of the neural IgCAM family, mediates anterior neural tube closure in a primitive chordate.Developmental biology · 2016Article
- Bisphenol-A induces expression of HOXC6, an estrogen-regulated homeobox-containing gene associated with breast cancer.Biochimica et biophysica acta · 2015Article
- Hox transcription factors: modulators of cell-cell and cell-extracellular matrix adhesion.BioMed research international · 2014Review
- Article
- Article
- HOXC6 is deregulated in human head and neck squamous cell carcinoma and modulates Bcl-2 expression.The Journal of biological chemistry · 2012Article
- HOX Gene Aberrant Expression in Skin Melanoma: A Review.Journal of skin cancer · 2012Article
- Environment and vascular bed origin influence differences in endothelial transcriptional profiles of coronary and iliac arteries.American journal of physiology. Heart and circulatory physiology · 2010Article
- Hox specificity unique roles for cofactors and collaborators.Current topics in developmental biology · 2009Review
- HOXD3-overexpression increases integrin alpha v beta 3 expression and deprives E-cadherin while it enhances cell motility in A549 cells.Clinical & experimental metastasis · 2006Article
- Histone deacetylase activity is essential for the expression of HoxA9 and for endothelial commitment of progenitor cells.The Journal of experimental medicine · 2005Article
- Hox genes and their candidate downstream targets in the developing central nervous system.Cellular and molecular neurobiology · 2005Review
- Article
- Article
- HOXC4, HOXC5, and HOXC6 expression in primary cutaneous lymphoid lesions. High expression of HOXC5 in anaplastic large-cell lymphomas.The American journal of pathology · 1997Article
- Barx2, a new homeobox gene of the Bar class, is expressed in neural and craniofacial structures during development.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- A binding site for Pax proteins regulates expression of the gene for the neural cell adhesion molecule in the embryonic spinal cord.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- HOXB7 constitutively activates basic fibroblast growth factor in melanomas.Molecular and cellular biology · 1996Article
- Embryonic expression patterns of the neural cell adhesion molecule gene are regulated by homeodomain binding sites.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Scores of homeobox gene-encoded transcription factors are expressed in a definite spatiotemporal pattern during embryogenesis and regulate a series of as yet unidentified target genes to help coordinate the morphogenetic process. We have suggested that homeobox gene products modulate the expression of adhesion molecule genes and have shown in cotransfection experiments that the promoters for the neural cell adhesion molecule (N-CAM) and cytotactin/tenascin genes respond to cues from different homeobox-containing genes. In this study, we show that the HoxC6 (Hox-3.3)-encoded homeoprotein binds to a DNA sequence in the N-CAM promoter CCTAATTATTAA, designated homeodomain binding site I (HBS-I). To test whether HoxC6 regulated N-CAM promoter activity, we cotransfected the Long and Short reading frame variants of Xenopus HoxC6 (CMV-HoxC6-L and CMV-HoxC6-S) driven by the human cytomegalovirus (CMV) promoter together with a chloramphenicol acetyltransferase (CAT) reporter gene driven by the mouse N-CAM promoter (N-CAM-Pro-CAT). Cotransfection of NIH 3T3 cells with either of the CMV-HoxC6 expression vectors stimulated N-CAM promoter-driven CAT expression. A 47-bp region from the N-CAM promoter that included HBS-I and an adjacent potential HBS, HBS-II, conferred HoxC6 regulation on a simian virus 40 minimal promoter. HBS-I was sufficient for transactivation of the minimal promoter by CMV-HoxC6-S. However, transcriptional activation by CMV-HoxC6-L required both HBS-I and HBS-II, inasmuch as mutation of either HBS-I, HBS-II, or both motifs abolished the response. These studies suggest that HBS-I is a target site for binding and transcriptional control of the N-CAM promoter by homeoproteins, although accessory DNA sequences (such as HBS-II) may also be required. Together with previous studies, these results support the notion that N-CAM gene expression may be controlled by different combinations of homeoproteins that appear in a place-dependent manner during embryogenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.