ArticleProceedings of the National Academy of Sciences of the United States of America1993
Generation of osteoclast-inductive and osteoclastogenic cell lines from the H-2KbtsA58 transgenic mouse.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 145 citations in OpenAlex.
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- Rice prolamin extract ameliorates acute murine colitis by inhibiting nuclear factor-kappa B and modulating intestinal apoptosis and cell proliferation.Clinical and experimental immunology · 2014Article
- Vitamin D endocrine system and osteoclasts.BoneKEy reports · 2014Article
- Historically significant events in the discovery of RANK/RANKL/OPG.World journal of orthopedics · 2013Review
- Carbon monoxide attenuates dextran sulfate sodium-induced colitis via inhibition of GSK-3β signaling.Oxidative medicine and cellular longevity · 2013Article
- Epigallocatechin-3-gallate Inhibits LPS-Induced NF-κB and MAPK Signaling Pathways in Bone Marrow-Derived Macrophages.Gut and liver · 2012Article
- Black tea extract prevents lipopolysaccharide-induced NF-κB signaling and attenuates dextran sulfate sodium-induced experimental colitis.BMC complementary and alternative medicine · 2011Article
- Evidence for osteocyte regulation of bone homeostasis through RANKL expression.Nature medicine · 2011Article
- Theaflavin Inhibits LPS-Induced IL-6, MCP-1, and ICAM-1 Expression in Bone Marrow-Derived Macrophages Through the Blockade of NF-κB and MAPK Signaling Pathways.Chonnam medical journal · 2011Article
- Immortalized CNS pericytes are quiescent smooth muscle actin-negative and pluripotent.Microvascular research · 2011Article
- Adenosine A1 receptors (A1Rs) play a critical role in osteoclast formation and function.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Mechano-transduction in osteoblastic cells involves strain-regulated estrogen receptor alpha-mediated control of insulin-like growth factor (IGF) I receptor sensitivity to Ambient IGF, leading to phosphatidylinositol 3-kinase/AKT-dependent Wnt/LRP5 receptor-independent activation of beta-catenin signaling.The Journal of biological chemistry · 2010Article
- A novel osteoclast precursor cell line, 4B12, recapitulates the features of primary osteoclast differentiation and function: enhanced transfection efficiency before and after differentiation.Journal of cellular physiology · 2009Article
- Characterization of fetal and postnatal enteric neuronal cell lines with improvement in intestinal neural function.Gastroenterology · 2008Article
- Contributions to osteoclast biology from Japan.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2008Review
- Age of donor alters the effect of cyclic hydrostatic pressure on production by human macrophages and osteoblasts of sRANKL, OPG and RANK.BMC musculoskeletal disorders · 2006Article
- Hybridoma-free generation of monoclonal antibodies.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Generation and characterization of a conditionally immortalized lung clara cell line from the H-2Kb-tsA58 transgenic mouse.In vitro cellular & developmental biology. Animal · 2002Article
- The molecular mechanism of osteoclastogenesis in rheumatoid arthritis.Arthritis research · 2002Review
- Regulation of osteoclast formation and function.Reviews in endocrine & metabolic disorders · 2001Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of osteoclastic cell lines would greatly facilitate analysis of the cellular and molecular biology of bone resorption. Several cell lines have previously been reported to be capable of osteoclastic differentiation. However, such cell lines form at best only occasional excavations, suggesting that osteoclastic differentiation is either incomplete or that osteoclasts represent a very small proportion of the cells present. We have used the recently developed H-2KbtsA58 transgenic mouse, in which the interferon-inducible major mouse histocompatibility complex H-2Kb promoter drives the temperature-sensitive (ts) immortalizing gene of simian virus 40 (tsA58), to develop cell lines from bone marrow with high efficiency. Bone marrow cells were incubated with gamma interferon at 33 degrees C, then cloned, and expanded. The cell lines were characterized at 39.5 degrees C in the absence of gamma interferon. First, stromal cell lines were established that induced osteclast formation (resorption of bone slices) when cocultured with hemopoietic spleen cells. Some of the stromal cell lines so generated were able to resorb approximately 30 mm2/cm2 of bone surface. We then established cell lines of hemopoietic origin, several of which possess osteoclastic potential. When these osteoclast-precursor cell lines were cocultured with stromal cell lines, extensive bone resorption was observed. Osteoclast formation did not occur if the precursor cell lines were incubated on bone slices without stromal cells; osteoclast formation was also dependent upon the presence of 1 alpha,25-dihydroxyvitamin D3. These cell lines represent a model for osteoclast formation and a valuable resource for identification of the mechanisms and factors that regulate osteoclast differentiation and function.
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