ArticleJournal of virology1993
Viral liposomes released from insect cells infected with recombinant baculovirus expressing the matrix protein of vesicular stomatitis virus.
Article in Journal of virology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 78 citations in OpenAlex.
- Development of innovative multi-epitope mRNA vaccine against central nervous system tuberculosis using in silico approaches.PloS one · 2024Article
- Human BST2 inhibits rabies virus release independently of cysteine-linked dimerization and asparagine-linked glycosylation.PloS one · 2023Article
- Design of multi-epitope vaccine candidate against Brucella type IV secretion system (T4SS).PloS one · 2023Article
- The two-stage interaction of Ebola virus VP40 with nucleoprotein results in a switch from viral RNA synthesis to virion assembly/budding.Protein & cell · 2022Article
- 18 Additional Amino Acids of the Signal Peptide of the Bombyx mori Nucleopolyhedrovirus GP64 Activates Immunoglobulin Binding Protein (BiP) Expression by RNA-seq Analysis.Current microbiology · 2021Article
- Accurately cleavable goat β-lactoglobulin signal peptide efficiently guided translation of a recombinant human plasminogen activator in transgenic rabbit mammary gland.Bioscience reports · 2019Article
- Article
- Arenavirus budding resulting from viral-protein-associated cell membrane curvature.Journal of the Royal Society, Interface · 2013Article
- Arenavirus reverse genetics for vaccine development.The Journal of general virology · 2013Article
- Cytopathogenesis of vesicular stomatitis virus is regulated by the PSAP motif of M protein in a species-dependent manner.Viruses · 2012Article
- Induction of cytopathic effects and apoptosis in Spodoptera frugiperda cells by the HIV-1 Env glycoprotein signal peptide.Virus genes · 2010Article
- Vesicular stomatitis virus matrix protein mutations that affect association with host membranes and viral nucleocapsids.The Journal of biological chemistry · 2009Article
- Roles for the recycling endosome, Rab8, and Rab11 in hantavirus release from epithelial cells.Virology · 2008Article
- Rhabdovirus matrix protein structures reveal a novel mode of self-association.PLoS pathogens · 2008Article
- Plasma membrane microdomains containing vesicular stomatitis virus M protein are separate from microdomains containing G protein and nucleocapsids.Journal of virology · 2008Article
- Mapping of the VP40-binding regions of the nucleoprotein of Ebola virus.Journal of virology · 2007Article
- The YLDL sequence within Sendai virus M protein is critical for budding of virus-like particles and interacts with Alix/AIP1 independently of C protein.Journal of virology · 2007Article
- Quantitative analysis of Nipah virus proteins released as virus-like particles reveals central role for the matrix protein.Virology journal · 2007Article
- Rabies virus matrix protein interplay with eIF3, new insights into rabies virus pathogenesis.Nucleic acids research · 2007Article
- Requirements for the assembly and release of Newcastle disease virus-like particles.Journal of virology · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The matrix (M) protein of vesicular stomatitis virus (VSV) has been found to promote assembly and budding of virions as well as down-regulating of VSV transcription. Large quantities of M protein can be produced in insect cells infected with recombinant baculovirus expressing the VSV M gene under control of the polyhedrin promoter. Analysis by pulse-chase experiments and density gradient centrifugation revealed that the [35S]methionine-labeled M protein synthesized in insect cells is released into the extracellular medium in association with lipid vesicles (liposomes). Electron microscopy and immunogold labeling showed that M protein expressed in insect cells induced the formation on plasma membrane of vesicles containing M protein, which are released from the cell surface in the form of liposomes. The baculovirus vector itself or recombinants expressing VSV glycoprotein (G) or nucleocapsid (N) protein did not produce the formation of vesicles in infected cells. The baculovirus-expressed M protein retains biological activity as demonstrated by its capacity to inhibit transcription when reconstituted with VSV nucleocapsids in vitro. These data suggest that M protein has the capacity to associate with the plasma membrane of infected cells and, in so doing, causes evagination of the membrane to form a vesicle which is released from the cell. This observation leads to the postulate, which requires further proof, that the VSV M protein can induce the formation and budding of liposomes from the cell membrane surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.