ArticleThe EMBO journal1993
Activation of tobacco retrotransposons during tissue culture.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 128 papers.
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Who cites it
128 citing papers in PubMed, 380 citations in OpenAlex.
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- Positive-Strand RNA Viruses Induce LTR Retrotransposon Transcription and Extrachromosomal Circular DNA Generation in Plants.International journal of molecular sciences · 2025Article
- Transposable elements as instructors of the immune system.Nature reviews. Immunology · 2025Review
- Clonal strains of the fresh-market potato cultivar Russet Norkotah changed the domestication gene CDF1.Plant physiology · 2025Article
- Breeding and biotechnology approaches to enhance the nutritional quality of rapeseed byproducts for sustainable alternative protein sources- a critical review.Frontiers in plant science · 2024Review
- Toward Transgene-Free Transposon-Mediated Biological Mutagenesis for Plant Breeding.International journal of molecular sciences · 2023Review
- Specific suppression of long terminal repeat retrotransposon mobilization in plants.Plant physiology · 2023Article
- Triggers and mediators of epigenetic remodeling in plants.Frontiers in epigenetics and epigenomics · 2023Review
- Identification and Characterization ofGenes · 2022Article
- Diverse and mobile: eccDNA-based identification of carrot low-copy-number LTR retrotransposons active in callus cultures.The Plant journal : for cell and molecular biology · 2022Article
- SunUp and Sunset genomes revealed impact of particle bombardment mediated transformation and domestication history in papaya.Nature genetics · 2022Article
- The Dynamism of Transposon Methylation for Plant Development and Stress Adaptation.International journal of molecular sciences · 2021Review
- GSNOR Contributes to Demethylation and Expression of Transposable Elements and Stress-Responsive Genes.Antioxidants (Basel, Switzerland) · 2021Article
- Genetic and epigenetic variation in transposable element expression responses to abiotic stress in maize.Plant physiology · 2021Article
- Datura genome reveals duplications of psychoactive alkaloid biosynthetic genes and high mutation rate following tissue culture.BMC genomics · 2021Article
- Extensively Current Activity of Transposable Elements in Natural Rice Accessions Revealed by Singleton Insertions.Frontiers in plant science · 2021Article
- Detection of mPing mobilization in transgenic rice plants.Genes & genomics · 2020Article
- Article
- Characterization of a heat-activated retrotransposon inBreeding science · 2018Article
68 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequences of at least three new families of retrotransposons (Tto1-Tto3) were amplified by PCR from cDNA prepared from protoplasts of an established tobacco cell line, based on the fact that certain amino acids are highly conserved in the reverse transcriptases encoded by retrotransposons. Structural analysis indicates that Tto1 is 5.5 kb long and has features typical of retrotransposons. Transcription of Tto1 starting in the long terminal repeat was active only in cultured cells. Protoplast formation enhanced the transcription. The copy number of Tto1 increased 10-fold in established cell lines; it also increased in plants regenerated from tissue cultures and in transgenic plants. These results indicate that Tto1 is activated during tissue culture. This is the first demonstration of activation of a plant retrotransposon by tissue culture. The copy number of Tto2 and a previously isolated transposon, Tnt1, also increased in established cell lines, indicating that these two retrotransposons may also be activated by tissue culture. These three retrotransposons are cryptic in normally propagated plants: no difference in the copy number was observed between individuals of the same cultivars or even between different cultivars.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.