ArticleThe Journal of physiology1993
Effects of amiloride and ouabain on short-chain fatty acid transport in guinea-pig large intestine.
Article in The Journal of physiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 61 citations in OpenAlex.
- Carbonic anhydrase influences asymmetric sodium and acetate transport across omasum of sheep.Animal bioscience · 2021Article
- Involvement of butyrate in electrogenic KPflugers Archiv : European journal of physiology · 2019Article
- pH gradients and a micro-pore filter at the luminal surface affect fluxes of propionic acid across guinea pig large intestine.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2007Article
- Propionate absorption associated with bicarbonate secretion in vitro in the mouse cecum.Pflugers Archiv : European journal of physiology · 2007Article
- Transport of acetate and sodium in sheep omasum: mutual, but asymmetric interactions.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2006Article
- Low carbon dioxide permeability of the apical epithelial membrane of guinea-pig colon.The Journal of physiology · 2005Article
- Effects of seasonal changes in food quality and food intake on the transport of sodium and butyrate across ruminal epithelium of reindeer.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2003Article
- The glow of the colonic pH microclimate kindled by short-chain fatty acids, chloride and bicarbonate.The Journal of physiology · 1999Review
- Maintenance and regulation of the pH microclimate at the luminal surface of the distal colon of guinea-pig.The Journal of physiology · 1999Article
- The characterization of butyrate transport across pig and human colonic luminal membrane.The Journal of physiology · 1998Article
- Mass spectrometric determination of HCO3- permeability and carbonic anhydrase activity in intact guinea-pig colon epithelium.The Journal of physiology · 1997Article
- Non-ionic diffusion and carrier-mediated transport drive extracellullar pH regulation of mouse colonic crypts.The Journal of physiology · 1996Article
- Article
- Metabolism of the macrolide immunosuppressant, tacrolimus, by the pig gut mucosa in the Ussing chamber.British journal of pharmacology · 1996Article
- Absorption of short-chain fatty acids across ruminal epithelium of sheep.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 1996Article
- An Na(+)-independent short-chain fatty acid transporter contributes to intracellular pH regulation in murine colonocytes.The Journal of general physiology · 1995Article
- Unidirectional fluxes of short-chain fatty acids across segments of the large intestine in pig, sheep and pony compared with guinea pig.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 1995Article
- Na(+)-, ouabain-, Ca(2+)-, and thapsigargin-sensitive ATPase activity expressed in chimeras between the calcium and the sodium pump alpha subunits.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Functional role of bicarbonate in propionate transport across guinea-pig isolated caecum and proximal colon.The Journal of physiology · 1994Article
- Basolateral mechanisms of intracellular pH regulation in the colonic epithelial cell line HT29 clone 19A.Pflugers Archiv : European journal of physiology · 1993Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Effects of H+ secreting mechanisms on unidirectional passage of the short-chain fatty acids (SCFA) acetate, propionate and n-butyrate across isolated guinea-pig caecum, proximal and distal colon were studied under short-circuit current conditions in Ussing chamber isotope flux experiments. 2. In the caecum and the proximal colon the serosal-to-mucosal fluxes (Jsm) were higher than the mucosal-to-serosal fluxes (Jms). Thus a net secretion of SCFA was present in the caecum and proximal colon. The higher Jsm appears to be coupled to the Na+ gradient established by the basolateral membrane Na(+)-K(+)-ATPase, whereas Jms is related to the operation of an apical membrane Na(+)-H+ exchanger. Inhibition of Na(+)-H+ exchange by amiloride (1 mM) added to the mucosal solution decreased Jms of SCFA in caecum and in proximal colon, but had no major effect in distal colon. 3. In distal colon Jms exceeds Jsm and thus a net absorption of SCFA was observed. Jms is Na+ independent and coupled to the activity of the apical membrane K(+)-H(+)-ATPase. Inhibition of the K(+)-H(+)-ATPase by addition of ouabain (0.1 mM) to the mucosal solution diminished Jms in the distal colon; in the caecum and proximal colon ouabain had no effect on Jms. 4. Neither amiloride nor ouabain caused major changes in Jsm in any of the large intestinal segments. 5. In conclusion, absorption of SCFA, e.g. Jms, in all large intestinal segments is related to the presence and activity of H+ secreting systems located in the apical membrane of colonocytes. In the caecum and proximal colon the predominant system appears to be Na(+)-H+ exchange, and in the distal colon K(+)-H(+)-ATPase. Supply of H+ ions allows protonation of SCFA anions and subsequent permeation by non-ionic diffusion across the apical membrane. These mechanisms account for 35, 40-50 and 60-80% of SCFA transport in the caecum, the proximal and the distal colon of guinea-pig, respectively. The nature of the Na(+)-dependent secretory pathway in the caecum and proximal colon remains to be determined.
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