ArticleThe Journal of cell biology1993
The role of calcium in the Chlamydomonas reinhardtii mating reaction.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- Chlamydomonas reinhardtii cellular compartments and their contribution to intracellular calcium signalling.Journal of experimental botany · 2021Review
- Species specific gene expression dynamics during harmful algal blooms.Scientific reports · 2020Article
- Gene Regulatory Networks for the Haploid-to-Diploid Transition ofPlant physiology · 2017Article
- Ciliary ectosomes: transmissions from the cell's antenna.Trends in cell biology · 2015Review
- Two calcium-dependent protein kinases from Chlamydomonas reinhardtii are transcriptionally regulated by nutrient starvation.Plant signaling & behavior · 2014Article
- Regulation of flagellar biogenesis by a calcium dependent protein kinase in Chlamydomonas reinhardtii.PloS one · 2013Article
- Gsp1 triggers the sexual developmental program including inheritance of chloroplast DNA and mitochondrial DNA in Chlamydomonas reinhardtii.The Plant cell · 2012Article
- Function and regulation of TRPP2 at the plasma membrane.American journal of physiology. Renal physiology · 2009Review
- Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella.The Journal of cell biology · 2007Article
- Genetics of the deflagellation pathway in Chlamydomonas.Genetics · 1998Article
- The Chlamydomonas mating type plus fertilization tubule, a prototypic cell fusion organelle: isolation, characterization, and in vitro adhesion to mating type minus gametes.The Journal of cell biology · 1997Article
- Cell adhesion-dependent inactivation of a soluble protein kinase during fertilization in Chlamydomonas.Molecular biology of the cell · 1996Article
- Signal transduction in the sexual life of Chlamydomonas.Plant molecular biology · 1994Review
- The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation.Molecular and cellular biology · 1994Article
- Flagellar adhesion-dependent regulation of Chlamydomonas adenylyl cyclase in vitro: a possible role for protein kinases in sexual signaling.The Journal of cell biology · 1994Article
- Two distinct, calcium-mediated, signal transduction pathways can trigger deflagellation in Chlamydomonas reinhardtii.The Journal of cell biology · 1994Article
- Activation of adenylyl cyclase in Chlamydomonas reinhardtii by adhesion and by heat.The Journal of cell biology · 1993Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The mating reaction of Chlamydomonas reinhardtii entails a rapid series of cell-cell interactions leading to cell fusion. We have demonstrated (Pasquale, S. M., and U. Goodenough. 1987. J. Cell Biol. 105:2279-2293) that cAMP plays a key role in this process: gametic flagellar adhesion elicits a sharp increase in intracellular cAMP, and presentation of dibutyryl-cAMP to unmated gametes elicits all known mating responses. The present study evaluates the role of Ca2+ in this system. We document that the mating-induced increase in cAMP, and hence the mating responses themselves, are blocked by a variety of drugs known to interfere with Ca(2+)-sensitive processes. These data suggest that Ca(2+)-mediated events may couple adhesion to the generation of cAMP. Such events, however, appear to be localized to the flagellar membrane; we find no evidence for the mating-related increase in cytosolic free Ca2+ that has been postulated by others. Indeed, by monitoring the length of the Ca(2+)-sensitive centrin-containing nucleus-basal body connector, we show that cytosolic free Ca2+ levels, if anything, decrease in response to cAMP signaling. We confirm a previous report that Ca2+ levels increase in the mating medium, but document that this represents a response to augmented cAMP levels and not a prelude. Finally, we show that IP3 levels remain constant throughout the mating reaction. These results are discussed in terms of the various signal transduction systems that have now been identified in Chlamydomonas.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.