ArticleThe Plant cell1993
Transposon-mediated mutations in the untranslated leader of maize Adh1 that increase and decrease pollen-specific gene expression.
Article in The Plant cell, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize.Nature communications · 2020Article
- Transposable elements: powerful contributors to angiosperm evolution and diversity.Genome biology and evolution · 2013Article
- Retrotransposon insertion into the maize waxy gene results in tissue-specific RNA processing.The Plant cell · 1997Article
- A transposable element in diverse corn lines, Ubiquitous (Uq): allelism test.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1995Article
- Features of the hmg 1 subfamily of genes encoding HMG-CoA reductase in potato.Plant molecular biology · 1995Article
- Transposon-induced promoter scrambling: a mechanism for the evolution of new alleles.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Promoter elements required for developmental expression of the maize Adh1 gene in transgenic rice.The Plant cell · 1994Article
- Plant viral leaders influence expression of a reporter gene in tobacco.Plant molecular biology · 1993Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The unstable mutation Adh1-Fm335 contains a Dissociation (Ds1) transposable element at position +53 in the untranslated leader of the maize Alcohol dehydrogenase-1 (Adh1) gene. Excision of Ds1 is known to generate new alleles with small additions and rearrangements of Adh1 DNA. We characterized 16 revertant alleles with respect to ADH1 activity levels in scutellum (nutritive tissue of the seed), anaerobic root, and pollen. Whereas gene expression was not different from the wild type in the sporophytic tissues of the scutellum and anaerobic root, there were strong allelic differences in pollen. One allele underexpressed pollen ADH1 at 48% of the wild-type level, and another overexpressed pollen ADH1 at 163% of the wild-type level. Quantitative RNase protection assays demonstrated that the mutant phenotypes reflected changes in the levels of steady state mRNA in pollen. These data provide a definitive demonstration of an overexpression mutant in plants and further show that marked increases in mRNA levels can follow minor alterations in central untranslated leader sequences. The nucleotide sequence of 12 new revertant alleles and the molecular mechanisms responsible for pollen-specific gene expression are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.