ArticleProceedings of the National Academy of Sciences of the United States of America1993
Direct coupling of opioid receptors to both stimulatory and inhibitory guanine nucleotide-binding proteins in F-11 neuroblastoma-sensory neuron hybrid cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 115 citations in OpenAlex.
- Delta opioid receptors engage multiple signaling cascades to differentially modulate prefrontal GABA release with input and target specificity.Cell reports · 2025Article
- Neuropeptide Y Y2 receptors in acute and chronic pain and itch.Neuropeptides · 2024Review
- Neuropeptide Y Y2 Receptors in Sensory Neurons Tonically Suppress Nociception and Itch but Facilitate Postsurgical and Neuropathic Pain Hypersensitivity.Anesthesiology · 2024Article
- Delta opioid receptors engage multiple signaling cascades to differentially modulate prefrontal GABA release with input and target specificity.bioRxiv : the preprint server for biology · 2024Article
- Immortalized Dorsal Root Ganglion Neuron Cell Lines.Frontiers in cellular neuroscience · 2020Review
- Opioid Activity in the Locus Coeruleus Is Modulated by Chronic Neuropathic Pain.Molecular neurobiology · 2019Article
- Lysophosphatidylinositol-induced activation of the cation channel TRPV2 triggers glucagon-like peptide-1 secretion in enteroendocrine L cells.The Journal of biological chemistry · 2017Article
- Mouse Neuroblastoma CBMethods in enzymology · 2017Article
- Activation of μ-opioid receptors inhibits calcium-currents in the vestibular afferent neurons of the rat through a cAMP dependent mechanism.Frontiers in cellular neuroscience · 2014Article
- Mu opioids and their receptors: evolution of a concept.Pharmacological reviews · 2013Review
- Pathophysiology of GPCR Homo- and Heterodimerization: Special Emphasis on Somatostatin Receptors.Pharmaceuticals (Basel, Switzerland) · 2012Article
- Ser/ Thr residues at α3/β5 loop of Gαs are important in morphine-induced adenylyl cyclase sensitization but not mitogen-activated protein kinase phosphorylation.The FEBS journal · 2012Article
- G protein-coupled oestrogen receptor 1 (GPER1)/GPR30: a new player in cardiovascular and metabolic oestrogenic signalling.British journal of pharmacology · 2011Review
- Subcellular localization of mu-opioid receptor G(s) signaling.The Journal of pharmacology and experimental therapeutics · 2010Article
- The calcium-sensing receptor couples to Galpha(s) and regulates PTHrP and ACTH secretion in pituitary cells.The Journal of endocrinology · 2010Article
- Switching of G-protein usage by the calcium-sensing receptor reverses its effect on parathyroid hormone-related protein secretion in normal versus malignant breast cells.The Journal of biological chemistry · 2008Article
- Sensitization of spinal cord nociceptive neurons with a conjugate of substance P and cholera toxin.BMC neuroscience · 2007Article
- G protein activation and cyclic AMP modulation by naloxone benzoylhydrazone in distinct layers of rat olfactory bulb.British journal of pharmacology · 2004Article
- Presynaptic inhibition of GABA(B)-mediated synaptic potentials in the ventral tegmental area during morphine withdrawal.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- L-Type calcium channels are required for one form of hippocampal mossy fiber LTP.Journal of neurophysiology · 1998Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Evidence is presented for linkage of opioid receptors directly to the stimulatory G protein (guanine nucleotide-binding protein), Gs, in addition to the generally accepted linkage to the inhibitory and "other" G proteins, gi and Go, in F-11 (neuroblastoma-dorsal root ganglion neuron) hybrid cells. Treatment of intact F-11 cells with cholera toxin decreased specific binding of the opioid agonist [D-Ala2,D-Leu5]enkephalin to F-11 cell membranes by 35%, with the remaining binding retaining high affinity for agonist. Under these conditions cholera toxin influenced the alpha subunit of Gs (Gs alpha) but had no effect on the alpha subunit of Gi/o (Gi/o alpha), based on ADP-ribosylation studies. Pertussis toxin treatment decreased high-affinity opioid agonist binding by about 50%; remaining binding was also of high affinity, even though pertussis toxin had inactivated Gi/o alpha selectively and essentially completely. Simultaneous treatment with both toxins had an additive effect, reducing specific binding by about 80%. While opioid agonists inhibited forskolin-stimulated adenylate cyclase activity of F-11 cells as expected, opioids also stimulated basal adenylate cyclase activity, indicative of interaction with Gs as well as Gi. Cholera toxin treatment attenuated opioid-stimulation of basal adenylate cyclase, whereas pertussis toxin treatment enhanced stimulation. In contrast, inhibition by opioid of forskolin-stimulated activity was attenuated by pertussis toxin but not by cholera toxin. It is concluded that a subset of opioid receptors may be linked directly to Gs and thereby mediate stimulation of adenylate cyclase. This Gs-adenylate cyclase interaction is postulated to be responsible for the novel excitatory electrophysiologic responses to opioids found in our previous studies of sensory neurons and F-11 cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.