ArticleIn vitro cellular & developmental biology : journal of the Tissue Culture Association1993
Characterization of the DiFi rectal carcinoma cell line derived from a familial adenomatous polyposis patient.
Article in In vitro cellular & developmental biology : journal of the Tissue Culture Association, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 46 citations in OpenAlex.
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- Copy number load predicts outcome of metastatic colorectal cancer patients receiving bevacizumab combination therapy.Nature communications · 2018Article
- An miRNA Expression Signature for the Human Colonic Stem Cell Niche Distinguishes Malignant from Normal Epithelia.Cancer research · 2017Article
- Antibody targeting of claudin-1 as a potential colorectal cancer therapy.Journal of experimental & clinical cancer research : CR · 2017Article
- Somatostatin signaling via SSTR1 contributes to the quiescence of colon cancer stem cells.BMC cancer · 2016Article
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- A novel in vitro assay to assess phosphorylation of 3'-[(18)F]fluoro-3'-deoxythymidine.Molecular imaging and biology · 2011Article
- Molecular imaging metrics to evaluate response to preclinical therapeutic regimens.Frontiers in bioscience (Landmark edition) · 2011Review
- Molecular imaging of therapeutic response to epidermal growth factor receptor blockade in colorectal cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2008Article
- Digital karyotyping.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
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- Expression of mucin-associated sulfo-Lea carbohydrate epitopes on human colon carcinoma cells.Japanese journal of cancer research : Gann · 1998Article
- Induction of cyclo-oxygenase-2 mRNA by prostaglandin E2 in human prostatic carcinoma cells.British journal of cancer · 1997Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The DiFi human colorectal cancer cell line was recently established from a familial adenomatous polyposis patient with extracolonic features characteristic of the Gardner syndrome. These cells have now been propagated for 150 passages in standard culture media and vessels without feeder layers or collagen coatings. They retain features of colonic epithelial cells such as surface microvilli, secretory vesicles, and desmosomes. Cytosol of DiFi cells contains a high level (502 U/mg protein) of the mucin CA 19-9. In addition, DiFi cells produce carcinoembryonic antigen, and induce tumors in athymic mice. Cytoskeleton analysis of DiFi cells by fluorescence microscopy showed a pronounced disorganization of actin cable structure. The isozyme genetic signature of DiFi cells is unique (0.01 probability of finding the same genetic signature in a different cell line), differs from that of HeLa cells, and has expressional features seen in other colorectal cell lines. The DiFi cell karyotype is tetraploid, contains many marker chromosomes, and shows numerous episomal particles. Two copies of chromosome 18 were absent, and only a single normal chromosome 17 was found. This parallels detection of allelic losses from DiFi cell DNA at loci on chromosomes 17p and 18 using molecular (cDNA) probes. DiFi cells clearly express transcripts for the c-myc proto-oncogene, the c-myb proto-oncogene, and the p53 tumor suppressor gene. Transforming growth factor beta inhibits DiFi cell growth in soft agar and suppresses c-myc expression in these cells. The value of this cell line in the study of genetic alterations in colorectal cancer is discussed.
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