ArticleGenetics1993
A novel mutation in DNA topoisomerase I of yeast causes DNA damage and RAD9-dependent cell cycle arrest.
Article in Genetics, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 72 citations in OpenAlex.
- A multispecies toolkit ofProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Genome Instability Induced by Topoisomerase Misfunction.International journal of molecular sciences · 2024Review
- SUMO: A Swiss Army Knife for Eukaryotic Topoisomerases.Frontiers in molecular biosciences · 2022Review
- Trapped topoisomerase II initiates formation of de novo duplications via the nonhomologous end-joining pathway in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Transcriptome Reprogramming of SymbiodiniaceaeFrontiers in physiology · 2020Article
- Genome-destabilizing effects associated with top1 loss or accumulation of top1 cleavage complexes in yeast.PLoS genetics · 2015Article
- A novel approach for organelle-specific DNA damage targeting reveals different susceptibility of mitochondrial DNA to the anticancer drugs camptothecin and topotecan.Nucleic acids research · 2009Article
- Mutation of Gly721 alters DNA topoisomerase I active site architecture and sensitivity to camptothecin.The Journal of biological chemistry · 2008Article
- Enhanced stimulation of chromosomal translocations by radiomimetic DNA damaging agents and camptothecin in Saccharomyces cerevisiae rad9 checkpoint mutants.Mutation research · 2004Article
- Article
- Recombinogenic flap ligation pathway for intrinsic repair of topoisomerase IB-induced double-strand breaks.Molecular and cellular biology · 2000Article
- Requirement for end-joining and checkpoint functions, but not RAD52-mediated recombination, after EcoRI endonuclease cleavage of Saccharomyces cerevisiae DNA.Molecular and cellular biology · 1998Article
- Mechanism of DNA transesterification by vaccinia topoisomerase: catalytic contributions of essential residues Arg-130, Gly-132, Tyr-136 and Lys-167.Nucleic acids research · 1997Article
- Identification of a mid-anaphase checkpoint in budding yeast.The Journal of cell biology · 1997Article
- Gene conversion plays the major role in controlling the stability of large tandem repeats in yeast.The EMBO journal · 1996Article
- Topoisomerase I involvement in illegitimate recombination in Saccharomyces cerevisiae.Molecular and cellular biology · 1996Article
- SCT1 mutants suppress the camptothecin sensitivity of yeast cells expressing wild-type DNA topoisomerase I.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Transcription, topoisomerases and recombination.Experientia · 1994Review
- Yeast Saccharomyces cerevisiae as a model system to study the cytotoxic activity of the antitumor drug camptothecin.Cancer chemotherapy and pharmacology · 1994Review
- RAD9-dependent G1 arrest defines a second checkpoint for damaged DNA in the cell cycle of Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
DNA topoisomerases, enzymes that alter the superhelicity of DNA, have been implicated in such critical cellular functions as transcription, DNA replication, and recombination. In the yeast Saccharomyces cerevisiae, a null mutation in the gene encoding topoisomerase I (TOP1) causes elevated levels of mitotic recombination in the ribosomal DNA (rDNA), but has little effect on growth. We have isolated a missense mutation in TOP1 that causes mitotic hyper-recombination not only in the rDNA, but also at other loci, in addition to causing a number of other unexpected phenotypes. This topoisomerase I mutation (top1-103) causes slow growth, constitutive expression of DNA damage-inducible genes, and inviability in the absence of the double-strand break repair system. Overexpression of top1-103 causes RAD9-dependent cell cycle arrest in G2. We show that the Top1-103 enzyme nicks DNA in vitro, suggesting that it damages DNA directly. We propose that Top1-103 mimics the action of wild-type topoisomerase I in the presence of the anti-tumor drug, camptothecin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.