ArticleJournal of neurochemistry1993
kappa-Opioid agonist modulation of [3H]thymidine incorporation into DNA: evidence for the involvement of pertussis toxin-sensitive G protein-coupled phosphoinositide turnover.
Article in Journal of neurochemistry, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- 30 years of dynorphins--new insights on their functions in neuropsychiatric diseases.Pharmacology & therapeutics · 2009Review
- Kappa opioids promote the proliferation of astrocytes via Gbetagamma and beta-arrestin 2-dependent MAPK-mediated pathways.Journal of neurochemistry · 2008Article
- Sciatic nerve ligation-induced proliferation of spinal cord astrocytes is mediated by kappa opioid activation of p38 mitogen-activated protein kinase.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Mu- and kappa-opioids induce the differentiation of embryonic stem cells to neural progenitors.The Journal of biological chemistry · 2006Article
- Differential mechanisms of morphine antinociceptive tolerance revealed in (beta)arrestin-2 knock-out mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Epidermal growth factor influenced by opioid peptides in immature rat uterus.Journal of endocrinological investigation · 2000Article
- Mu-opioid agonist inhibition of kappa-opioid receptor-stimulated extracellular signal-regulated kinase phosphorylation is dynamin-dependent in C6 glioma cells.Journal of neurochemistry · 2000Article
- Mitogenic signaling via endogenous kappa-opioid receptors in C6 glioma cells: evidence for the involvement of protein kinase C and the mitogen-activated protein kinase signaling cascade.Journal of neurochemistry · 2000Article
- Evidence for kappa- and mu-opioid receptor expression in C6 glioma cells.Journal of neurochemistry · 1998Article
- Opioid modulation of extracellular signal-regulated protein kinase activity is ras-dependent and involves Gbetagamma subunits.Journal of neurochemistry · 1998Article
- mu-Opioid receptors modulate NMDA receptor-mediated responses in nucleus accumbens neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Stimulatory effects of opioids on transmitter release and possible cellular mechanisms: overview and original results.Neurochemical research · 1996Review
- kappa-opioid receptor expression defines a phenotypically distinct subpopulation of astroglia: relationship to Ca2+ mobilization, development, and the antiproliferative effect of opioids.Brain research · 1996Article
- kappa opioid receptors in human microglia downregulate human immunodeficiency virus 1 expression.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- mu-Opioid receptor-induced Ca2+ mobilization and astroglial development: morphine inhibits DNA synthesis and stimulates cellular hypertrophy through a Ca(2+)-dependent mechanism.Brain research · 1996Article
- Dynorphins modulate DNA synthesis in fetal brain cell aggregates.Journal of neurochemistry · 1995Article
- Opioids inhibit endothelin-mediated DNA synthesis, phosphoinositide turnover, and Ca2+ mobilization in rat C6 glioma cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1994Article
- Beta-endorphin is a potent inhibitor of thymidine incorporation into DNA via mu- and kappa-opioid receptors in fetal rat brain cell aggregates in culture.Journal of neurochemistry · 1993Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
A body of evidence has indicated that mu-opioid agonists can inhibit DNA synthesis in developing brain. We now report that kappa-selective opioid agonists (U69593 and U50488) modulate [3H]thymidine incorporation into DNA in fetal rat brain cell aggregates in a dose- and developmental stage-dependent manner, kappa agonists decreased thymidine incorporation by 35% in cultures grown for 7 days, and this process was reversed by the kappa-selective antagonist, norbinaltorphimine, whereas in 21-day brain cell aggregates a 3.5-fold increase was evident. Cell labeling by [3H]thymidine was also inhibited by the kappa-opioid agonist as shown by autoradiography. In addition, U69593 reduced basal rates of phosphoinositide formation in 7-day cultures and elevated it in 21-day cultures. Control levels were restored by norbinaltorphimine. Pertussis toxin blocked U69593-mediated inhibition of DNA synthesis. The action of kappa agonists on thymidine incorporation in the presence of chelerythrine, a protein kinase C (PKC) inhibitor, or in combination with LiCl, a noncompetitive inhibitor of inositol phosphatase, was attenuated in both 7- and 21-day cultures. These results suggest that kappa agonists may inhibit DNA synthesis via the phosphoinositide system with a pertussis toxin-sensitive G protein as transducer. In mixed glial cell aggregates, U50488 increased thymidine incorporation into DNA 3.1-fold, and this stimulation was reversed by the opioid antagonist naltrexone.
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