ArticleProceedings of the National Academy of Sciences of the United States of America1993
Electrical activity-dependent regulation of the acetylcholine receptor delta-subunit gene, MyoD, and myogenin in primary myotubes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- In Vitro Innervation as an Experimental Model to Study the Expression and Functions of Acetylcholinesterase and Agrin in Human Skeletal Muscle.Molecules (Basel, Switzerland) · 2017Review
- A system for studying mechanisms of neuromuscular junction development and maintenance.Development (Cambridge, England) · 2016Article
- In vitro Differentiation of Functional Human Skeletal Myotubes in a Defined System.Biomaterials science · 2014Article
- Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons.Developmental biology · 2011Article
- A histone deacetylase 4/myogenin positive feedback loop coordinates denervation-dependent gene induction and suppression.Molecular biology of the cell · 2009Article
- MyoD- and nerve-dependent maintenance of MyoD expression in mature muscle fibres acts through the DRR/PRR element.BMC developmental biology · 2008Article
- Developmental expression of the TTX-resistant voltage-gated sodium channels Nav1.8 (SNS) and Nav1.9 (SNS2) in primary sensory neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Activation and cellular localization of the cyclosporine A-sensitive transcription factor NF-AT in skeletal muscle cells.Molecular biology of the cell · 1998Article
- Spontaneous activity regulates calcium-dependent K+ current expression in developing ascidian muscle.The Journal of physiology · 1998Article
- Cross-talk between transcriptional regulation by thyroid hormone and myogenin: new aspects of the Ca2+-dependent expression of the fast-type sarcoplasmic reticulum Ca2+-ATPase.The Biochemical journal · 1998Article
- Effect of atrophy and contractions on myogenin mRNA concentration in chick and rat myoblast omega muscle cells.In vitro cellular & developmental biology. Animal · 1997Article
- Co-ordinated modulation of Ca2+ and K+ currents during ascidian muscle development.The Journal of physiology · 1996Article
- Modifications of current properties by expression of a foreign potassium channel gene in Xenopus embryonic cells.The Journal of membrane biology · 1995Article
- Adaptation of nicotinic acetylcholine receptor, myogenin, and MRF4 gene expression to long-term muscle denervation.The Journal of cell biology · 1995Article
- In vivo and in vitro analysis of electrical activity-dependent expression of muscle acetylcholine receptor genes using adenovirus.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Rapid inhibition of myogenin-driven acetylcholine receptor subunit gene transcription.The EMBO journal · 1994Article
- Immunohistochemical analysis of the distribution of MyoD1 in muscle biopsies of primary myopathies and neurogenic atrophy.Acta neuropathologica · 1994Article
- Jun, Fos, MyoD1, and myogenin proteins are increased in skeletal muscle fiber nuclei after denervation.Acta neuropathologica · 1994Article
- Upstream sequences of the myogenin gene convey responsiveness to skeletal muscle denervation in transgenic mice.Nucleic acids research · 1993Article
- Marked, transient inhibition of expression of the Epstein-Barr virus latent membrane protein gene in Burkitt's lymphoma cell lines by electroporation.Journal of virology · 1993Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Expression of the skeletal muscle acetylcholine receptor (AChR) is regulated by nerve-evoked muscle activity. Studies using transgenic mice have shown that this regulation is controlled largely by transcriptional mechanisms because responsiveness to electrical activity can be conferred by transgenes containing cis-acting sequences from the AChR subunit genes. The lack of a convenient muscle cell culture system for studying electrical activity-dependent gene regulation, however, has made it difficult to identify the important cis-acting sequences and to characterize an electrical activity-dependent signaling pathway. We developed a muscle culture system to study the mechanisms of electrical activity-dependent gene expression. Gene fusions between the murine AChR delta-subunit gene and the human growth hormone gene were transfected into primary myoblasts, and the amount of growth hormone secreted into the culture medium from either spontaneously electrically active or inactive myotube cultures was measured. We show that 181 bp of 5'-flanking DNA from the AChR delta-subunit gene are sufficient to confer electrical activity-dependent gene expression. In addition, we show that the rate of AChR delta-subunit gene expression differs among individual nuclei in a single myotube but that highly expressing nuclei are not necessarily colocalized with AChR clusters. We also show that expression of MyoD and myogenin are regulated by electrical activity in primary myotube cultures and that all nuclei within a myotube express similar levels of MyoD and similar levels of myogenin.
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