ArticleMolecular biology of the cell1993
The unconventional myosin encoded by the myoA gene plays a role in Dictyostelium motility.
Article in Molecular biology of the cell, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 147 citations in OpenAlex.
- One must reconstitute the functions of interest from purified proteins.Frontiers in physiology · 2024Review
- Class I Myosins, molecular motors involved in cell migration and cancer.Cell adhesion & migration · 2022Article
- Selective localization of myosin-I proteins in macropinosomes and actin waves.Cytoskeleton (Hoboken, N.J.) · 2016Article
- Structure of the small Dictyostelium discoideum myosin light chain MlcB provides insights into MyoB IQ motif recognition.The Journal of biological chemistry · 2014Article
- PakB binds to the SH3 domain of Dictyostelium Abp1 and regulates its effects on cell polarity and early development.Molecular biology of the cell · 2013Article
- 'Dicty dynamics': Dictyostelium motility as persistent random motion.Physical biology · 2011Article
- Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites.The EMBO journal · 2010Article
- Leveraging the membrane - cytoskeleton interface with myosin-1.Trends in cell biology · 2010Review
- Unconventional myosins acting unconventionally.Trends in cell biology · 2009Review
- Myosin-1c couples assembling actin to membranes to drive compensatory endocytosis.Developmental cell · 2006Article
- Cell motility and local viscoelasticity of fibroblasts.Biophysical journal · 2005Article
- The Dictyostelium class I myosin, MyoD, contains a novel light chain that lacks high-affinity calcium-binding sites.The Biochemical journal · 2003Article
- Functions of LIM proteins in cell polarity and chemotactic motility.The EMBO journal · 2002Article
- Myosin 1c and myosin IIB serve opposing roles in lamellipodial dynamics of the neuronal growth cone.The Journal of cell biology · 2002Article
- Tortoise, a novel mitochondrial protein, is required for directional responses of Dictyostelium in chemotactic gradients.The Journal of cell biology · 2001Article
- A myosin I is involved in membrane recycling from early endosomes.The Journal of cell biology · 2000Article
- The internal phosphodiesterase RegA is essential for the suppression of lateral pseudopods during Dictyostelium chemotaxis.Molecular biology of the cell · 2000Article
- Intracellular growth of Legionella pneumophila in Dictyostelium discoideum, a system for genetic analysis of host-pathogen interactions.Infection and immunity · 2000Article
- Direct involvement of yeast type I myosins in Cdc42-dependent actin polymerization.The Journal of cell biology · 2000Article
- Myosin I contributes to the generation of resting cortical tension.Biophysical journal · 1999Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The myoA gene of Dictyostelium is a member of a gene family of unconventional myosins. The myosin Is share homologous head and basic domains, but the myoA gene product lacks the glycine-, proline-, alanine-rich and src homology 3 domains typical of several of the other myosin Is. A mutant strain of Dictyostelium lacking a functional myoA gene was produced by gene targeting, and the motility of this strain in buffer and a spatial gradient of the chemoattractant cyclic AMP was analyzed by computer-assisted methods. The myoA- cells have a normal elongate morphology in buffer but exhibit a decrease in the instantaneous velocity of cellular translocation, an increase in the frequency of lateral pseudopod formation, and an increase in turning. In a spatial gradient, in which the frequency of pseudopod formation is depressed, myoA- cells exhibit positive chemotaxis but still turn several times more frequently than control cells. These results demonstrate that the other members of the unconventional myosin family do not fully compensate for the loss of functional myoA gene product. Surprisingly, the phenotype of the myoA- strain closely resembles that of the myoB- strain, suggesting that both play a role in the frequency of pseudopod formation and turning during cellular translocation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.