ArticleNucleic acids research1993
Regulation of the mouse desmin gene: transactivated by MyoD, myogenin, MRF4 and Myf5.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 102 citations in OpenAlex.
- Increased p70s6k phosphorylation during intake of a protein-carbohydrate drink following resistance exercise in the fasted state.European journal of applied physiology · 2010Trial
- Molecular insights into the role of desmin intermediate filament network in chromatin landscape, cardiomyocyte differentiation, and maturation.Cell death & disease · 2025Article
- Article
- Desmin deficiency affects the microenvironment of the cardiac side population and Sca1Cell and tissue research · 2022Article
- Transcription Regulation ofBiology · 2022Article
- IL-4 and SDF-1 Increase Adipose Tissue-Derived Stromal Cell Ability to Improve Rat Skeletal Muscle Regeneration.International journal of molecular sciences · 2020Article
- Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568.Journal of fluorescence · 2020Article
- A Muscle Hybrid Promoter as a Novel Tool for Gene Therapy.Molecular therapy. Methods & clinical development · 2019Article
- Intermediate filaments in cardiomyopathy.Biophysical reviews · 2018Review
- Enhanced Development of Skeletal Myotubes from Porcine Induced Pluripotent Stem Cells.Scientific reports · 2017Article
- Spatial re-organization of myogenic regulatory sequences temporally controls gene expression.Nucleic acids research · 2015Article
- Posttranslational modifications of desmin and their implication in biological processes and pathologies.Histochemistry and cell biology · 2014Review
- Role of nitric oxide in muscle regeneration following eccentric muscle contractions in rat skeletal muscle.The journal of physiological sciences : JPS · 2013Article
- Mechano growth factor (MGF) promotes proliferation and inhibits differentiation of porcine satellite cells (PSCs) by down-regulation of key myogenic transcriptional factors.Molecular and cellular biochemistry · 2012Article
- SREBP-1 transcription factors regulate skeletal muscle cell size by controlling protein synthesis through myogenic regulatory factors.PloS one · 2012Article
- Myogenic potential of whole bone marrow mesenchymal stem cells in vitro and in vivo for usage in urinary incontinence.PloS one · 2012Article
- Sequential association of myogenic regulatory factors and E proteins at muscle-specific genes.Skeletal muscle · 2011Article
- A new role for sterol regulatory element binding protein 1 transcription factors in the regulation of muscle mass and muscle cell differentiation.Molecular and cellular biology · 2010Article
- Myogenic regulatory factor response to resistance exercise volume in skeletal muscle.European journal of applied physiology · 2010Article
- S. macrurus myogenic regulatory factors (MRFs) induce mammalian skeletal muscle differentiation; evidence for functional conservation of MRFs.The International journal of developmental biology · 2009Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Desmin, the muscle specific intermediate filament (IF) protein, is expressed at low levels in myoblasts and at the onset of differentiation its expression increases several fold. In an effort to explore the mechanism involved in the tissue-specific and developmentally regulated expression of desmin, we have isolated the mouse desmin gene. Sequence analysis of 976 bp 5' flanking region revealed several potential cis-acting elements: 1) Three E boxes (MyoD binding sites), namely, E1, E2 and E3, located at -79, -832 and -936, respectively; 2) one MEF2 binding site at -864; 3) a region with homology to M-CAT motif at -587; 4) several GC boxes. Transient transfections with various 5' flank deletion mutants into C2C12 muscle cells have revealed both positive and negative elements that seem to be involved in the expression of desmin. The first 81 bp upstream of the transcription initiation site, including E1 box, were sufficient to confer muscle specific expression of the desmin gene. The maximal level of expression was achieved by the construct containing up to -897 base pairs. The region between -578 to -976 behaves as a classical enhancer in the absence of which the region between -578 and -81 suppresses CAT activity. Gel electrophoretic mobility shift assays using both C2C12 muscle cell nuclear extracts as well as in vitro translated myoD/E12 and myogenin/E12 heterodimers, showed that both myoD and myogenin bind to the proximal E1 and the distal E2 boxes of the desmin promoter and enhancer respectively. Co-transfection of myoD, myogenin, MRF4 and Myf5, with the desmin-CAT construct into 10T-1/2 cells demonstrated that all these factors could transactivate desmin gene expression.
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