ArticleNucleic acids research1993
Facile preparation of nuclease resistant 3' modified oligodeoxynucleotides.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 109 citations in OpenAlex.
- Inhibition of transcription and antiproliferative effects in a cancer cell line using antigene oligonucleotides containing artificial nucleoside analogues.RSC medicinal chemistry · 2023Article
- Development of Phosphoramidite Reagents for the Synthesis of Base-Labile Oligonucleotides Modified with a Linear Aminoalkyl and Amino-PEG Linker at the 3'-End.Molecules (Basel, Switzerland) · 2022Article
- Oxidation ofInternational journal of molecular sciences · 2022Article
- RNA-Cholesterol Nanoparticles Function as Potent Immune ActivatorsFrontiers in immunology · 2021Article
- Refilling drug delivery depots through the blood.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Design and synthesis of bis-biotin-containing reagents for applications utilizing monoclonal antibody-based pretargeting systems with streptavidin mutants.Bioconjugate chemistry · 2010Article
- Reagents for astatination of biomolecules. 3. Comparison of closo-decaborate(2-) and closo-dodecaborate(2-) moieties as reactive groups for labeling with astatine-211.Bioconjugate chemistry · 2009Article
- Selective glutaraldehyde-mediated coupling of proteins to the 3'-adenine terminus of polymerase chain reaction products.Journal of biomolecular techniques : JBT · 2008Article
- Characterization of aberrant melting peaks in unlabeled probe assays.The Journal of molecular diagnostics : JMD · 2007Article
- Inhibitory effect of ribbon-type NF-kappaB decoy oligodeoxynucleotides on osteoclast induction and activity in vitro and in vivo.Arthritis research & therapy · 2006Article
- 3'-Exonuclease resistance of DNA oligodeoxynucleotides containing O6-[4-oxo-4-(3-pyridyl)butyl]guanine.Nucleic acids research · 2003Article
- Reduced aggregation and improved specificity of G-rich oligodeoxyribonucleotides containing pyrazolo[3,4-d]pyrimidine guanine bases.Nucleic acids research · 2002Article
- 3'-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures.Nucleic acids research · 2000Article
- Atomic force microscopy imaging of DNA covalently immobilized on a functionalized mica substrate.Biophysical journal · 1999Article
- Real time detection of DNA.RNA hybridization in living cells.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Triplex targeting of a native gene in permeabilized intact cells: covalent modification of the gene for the chemokine receptor CCR5.Nucleic acids research · 1998Article
- Factors influencing the extent and selectivity of alkylation within triplexes by reactive G/A motif oligonucleotides.Nucleic acids research · 1997Article
- Sequence-specific targeting and covalent modification of human genomic DNA.Nucleic acids research · 1997Article
- Targeting hepatitis B therapy to the liver. Clinical pharmacokinetic considerations.Clinical pharmacokinetics · 1996Review
- Sequence-specific arrest of primer extension on single-stranded DNA by an oligonucleotide-minor groove binder conjugate.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An efficient chemical procedure for the immobilization of carboxylate containing conjugate groups onto controlled pore glass (CPG) is described. The derivatized supports were used in the automated synthesis of an oligodeoxynucleotide (20-mer ODN) containing a 3' phosphodiester linked hexanol, aminohexyl, acridine, or cholesterol group. The stability of the oligomer in a hepatoma cell culture was found to be prolonged two to three fold by the presence of any of the 3' tails. By contrast, an aminohexyl group appended to the 5' terminus of the ODN only marginally improved its nuclease resistance. These data support the notion that antisense ODNs are primarily degraded by 3' exonucleases. Introduction of simple 3' tails which incorporate a normal phosphodiester linkage can increase ODN stability by interfering with these enzymes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.