ArticleThe EMBO journal1993
Muscle-specific expression of the acetylcholine receptor alpha-subunit gene requires both positive and negative interactions between myogenic factors, Sp1 and GBF factors.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 62 citations in OpenAlex.
- Emerging parallels between stomatal and muscle cell lineages.Plant physiology · 2009Article
- Sp1 and AP-1 regulate expression of the human gene VIL2 in esophageal carcinoma cells.The Journal of biological chemistry · 2009Article
- Inferring biological functions and associated transcriptional regulators using gene set expression coherence analysis.BMC bioinformatics · 2007Article
- Myogenic basic helix-loop-helix proteins and Sp1 interact as components of a multiprotein transcriptional complex required for activity of the human cardiac alpha-actin promoter.Molecular and cellular biology · 1999Article
- Neu differentiation factor stimulates phosphorylation and activation of the Sp1 transcription factor.Molecular and cellular biology · 1999Article
- Article
- Implication of a multisubunit Ets-related transcription factor in synaptic expression of the nicotinic acetylcholine receptor.The EMBO journal · 1998Article
- Characterization of human involucrin promoter distal regulatory region transcriptional activator elements-a role for Sp1 and AP1 binding sites.The Biochemical journal · 1998Article
- Combinatorial control of muscle development by basic helix-loop-helix and MADS-box transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 1996Review
- Identification and characterization of a 47 base pair activity-dependent enhancer of the rat nicotinic acetylcholine receptor delta-subunit promoter.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- A compilation of composite regulatory elements affecting gene transcription in vertebrates.Nucleic acids research · 1995Article
- A negative regulatory element in the promoter region of the rat alpha 2A-adrenergic receptor gene overlaps an SP1 consensus binding site.The Biochemical journal · 1995Article
- An E box in the desmin promoter cooperates with the E box and MEF-2 sites of a distal enhancer to direct muscle-specific transcription.The EMBO journal · 1994Article
- Identification of human T-cell lymphotropic virus type I 21-base-pair repeat-specific and glial cell-specific DNA-protein complexes.Journal of virology · 1994Article
- Promoter elements and transcriptional control of the chicken tropomyosin gene [corrected].Nucleic acids research · 1994Article
- Oligo[d(C).(G)] runs exhibit a helical repeat of 11.1 bp in solution and cause slight DNA curvature when properly phased.Nucleic acids research · 1994Article
- In vivo and in vitro analysis of electrical activity-dependent expression of muscle acetylcholine receptor genes using adenovirus.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- MyoD and myogenin act on the chicken myosin light-chain 1 gene as distinct transcriptional factors.Molecular and cellular biology · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dependence of the muscle-specific enhancer of the acetylcholine receptor alpha-subunit gene on other domains of the promoter has been analysed by performing point mutagenesis and modular reconstitution of the enhancer--promoter sequences. The enhancer is inactive in the absence of the proximal region containing an Sp1 binding site and an overlapping G-C homopolymer binding factor site (referred to as GBF). The proximal region can be replaced by an Sp1 binding site from SV40 or an MEF-2 binding site from the muscle creatine kinase gene. Specific mutation of the Sp1 site markedly affects transactivation by CMD1 or myogenin. Mutation of the GBF binding site leads to higher promoter activity in primary cultures of chick myotubes or in quail fibroblasts. In addition, binding of a purified Sp1 protein prevents the binding of GBF in vitro. It is proposed that in the case of the alpha-subunit promoter, the myogenic factors activate transcription in cooperation with Sp1, and that GBF contributes to muscle-specific expression of the promoter by interfering with Sp1 binding in nonmuscle muscle cells or myoblasts.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.