ArticleThe EMBO journal1993
A model for chromatin opening: stimulation of topoisomerase II and restriction enzyme cleavage of chromatin by distamycin.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 119 citations in OpenAlex.
- HMGA Proteins in Hematological Malignancies.Cancers · 2020Review
- Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells.BMC medical genomics · 2019Article
- Cancer cells' epigenetic composition and predisposition to histone deacetylase inhibitor sensitization.Epigenomics · 2011Review
- Article
- Functional characterization of a tobacco matrix attachment region-mediated enhancement of transgene expression.Transgenic research · 2009Article
- Nuclear condensation and free radical scavenging: a dual mechanism of bisbenzimidazoles to modulate radiation damage to DNA.Molecular and cellular biochemistry · 2007Article
- Chromatin as a target for the DNA-binding anticancer drugs.Sub-cellular biochemistry · 2007Review
- Isolation and characterization of SATB2, a novel AT-rich DNA binding protein expressed in development- and cell-specific manner in the rat brain.Neurochemical research · 2006Article
- The preferential binding of histone H1 to DNA scaffold-associated regions is determined by its C-terminal domain.Nucleic acids research · 2004Article
- Matrix attachment regions enhance transcription of a downstream transgene and the accessibility of its promoter region to micrococcal nuclease.Plant molecular biology · 2003Article
- The role of cell differentiation state and HMG-I/Y in the expression of transgenes flanked by matrix attachment regions.Transgenic research · 2001Article
- Regulation of matrix attachment region-dependent, lymphocyte-restricted transcription through differential localization within promyelocytic leukemia nuclear bodies.The EMBO journal · 2000Article
- Use of matrix attachment regions (MARs) to minimize transgene silencing.Plant molecular biology · 2000Review
- Chromatin condensation is confined to the loop and involves an all-or-none structural change.Biophysical journal · 1999Article
- Gadd45, a p53-responsive stress protein, modifies DNA accessibility on damaged chromatin.Molecular and cellular biology · 1999Article
- Attachment to the nuclear matrix mediates specific alterations in chromatin structure.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- In vivo analysis of scaffold-associated regions in Drosophila: a synthetic high-affinity SAR binding protein suppresses position effect variegation.The EMBO journal · 1998Article
- Contrasting effects of alpha and beta globin regulatory elements on chromatin structure may be related to their different chromosomal environments.The EMBO journal · 1995Article
- SARs stimulate but do not confer position independent gene expression.Nucleic acids research · 1994Article
- Binding of matrix attachment regions to lamin polymers involves single-stranded regions and the minor groove.Molecular and cellular biology · 1994Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Histone H1 preferentially and cooperatively binds scaffold-associated regions (SARs) in vitro via specific interactions with the numerous short A + T-rich tracts (A-tracts) contained in these sequences. Selective titration of A-tracts by the oligopeptide distamycin abolishes this interaction and results in a redistribution of H1. Similarly, treatment of intact cells and isolated nuclei with distamycin specifically enhances cleavage of internucleosomal linkers of SARs by topoisomerase II and restriction enzymes. The increased accessibility of these linkers is thought to result from the unfolding (or opening) of the chromatin fiber and to be due to a reduced occupancy by histone H1. Chromatin extraction and H1 assembly experiments support this view. We discuss a model whereby open, H1-depleted chromatin regions may be generated by titration of A-tracts by putative distamycin analogues; this local opening may spread to adjacent regions assuming highly cooperative H1-H1 interactions in chromatin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.