ArticleMolecular and cellular biology1993
Site-selected insertion of the transposon Tc1 into a Caenorhabditis elegans myosin light chain gene.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 90 citations in OpenAlex.
- Maternal effects on postembryonic neuroblast migration in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2025Article
- Transposons As Tools for Functional Genomics in Vertebrate Models.Trends in genetics : TIG · 2017Review
- Comprehensive functional genomics using Caenorhabditis elegans as a model organism.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2017Article
- Article
- Sleeping Beauty transposon mutagenesis of the rat genome in spermatogonial stem cells.Methods (San Diego, Calif.) · 2011Article
- Identification of transcription start sites of trans-spliced genes: uncovering unusual operon arrangements.RNA (New York, N.Y.) · 2011Article
- Dissection of genetic pathways in C. elegans.Methods in cell biology · 2011Review
- Article
- Article
- Transposon-mediated genome manipulation in vertebrates.Nature methods · 2009Review
- Caenorhabditis elegans: an emerging model in biomedical and environmental toxicology.Toxicological sciences : an official journal of the Society of Toxicology · 2008Review
- Efficient target-selected mutagenesis in Caenorhabditis elegans: toward a knockout for every gene.Genome research · 2007Article
- Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives.Genome biology · 2007Review
- Homologous gene targeting in Caenorhabditis elegans by biolistic transformation.Nucleic acids research · 2004Article
- Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR.Nucleic acids research · 2002Article
- Improved detection of small deletions in complex pools of DNA.Nucleic acids research · 2002Article
- Caenorhabditis elegans DNA mismatch repair gene msh-2 is required for microsatellite stability and maintenance of genome integrity.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Rabphilin potentiates soluble N-ethylmaleimide sensitive factor attachment protein receptor function independently of rab3.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Tc8, a Tourist-like transposon in Caenorhabditis elegans.Genetics · 2001Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We used the polymerase chain reaction to detect insertions of the transposon Tc1 into mlc-2, one of two Caenorhabditis elegans regulatory myosin light chain genes. Our goals were to develop a general method to identify mutations in any sequenced gene and to establish the phenotype of mlc-2 loss-of-function mutants. The sensitivity of the polymerase chain reaction allowed us to identify nematode populations containing rare Tc1 insertions into mcl-2. mlc-2::Tc1 mutants were subsequently isolated from these populations by a sib selection procedure. We isolated three mutants with Tc1 insertions within the mlc-2 third exon and a fourth strain with Tc1 inserted in nearby noncoding DNA. To demonstrate the generality of our procedure, we isolated two additional mutants with Tc1 insertions within hlh-1, the C. elegans MyoD homolog. All of these mutants are essentially wild type when homozygous. Despite the fact that certain of these mutants have Tc1 inserted within exons of the target gene, these mutations may not be true null alleles. All three of the mlc-2 mutants contain mlc-2 mRNA in which all or part of Tc1 is spliced from the pre-mRNA, leaving small in-frame insertions or deletions in the mature message. There is a remarkable plasticity in the sites used to splice Tc1 from these mlc-2 pre-mRNAs; certain splice sites used in the mutants are very different from typical eukaryotic splice sites.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.