ArticleMolecular and cellular biology1993
A mobile group I intron from Physarum polycephalum can insert itself and induce point mutations in the nuclear ribosomal DNA of saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 61 citations in OpenAlex.
- Article
- Modeling in yeast how rDNA introns slow growth and increase desiccation tolerance in lichens.G3 (Bethesda, Md.) · 2021Article
- Nucleolar release of rDNA repeats for repair involves SUMO-mediated untethering by the Cdc48/p97 segregase.Nature communications · 2021Article
- Phenotypic and Genotypic Consequences of CRISPR/Cas9 Editing of the Replication Origins in the rDNA ofGenetics · 2019Article
- Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.Microbial cell (Graz, Austria) · 2019Review
- RNase H eliminates R-loops that disrupt DNA replication but is nonessential for efficient DSB repair.EMBO reports · 2018Article
- Vector control with driving Y chromosomes: modelling the evolution of resistance.Malaria journal · 2017Article
- Article
- Nuclear group I introns in self-splicing and beyond.Mobile DNA · 2013Article
- A new method to efficiently induce a site-specific double-strand break in the fission yeast Schizosaccharomyces pombe.Yeast (Chichester, England) · 2012Article
- Homing endonucleases: from basics to therapeutic applications.Cellular and molecular life sciences : CMLS · 2010Review
- Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease.Nucleic acids research · 2007Article
- Spontaneous rDNA copy number variation modulates Sir2 levels and epigenetic gene silencing.Genes & development · 2005Article
- Mobile self-splicing group I introns from the psbA gene of Chlamydomonas reinhardtii: highly efficient homing of an exogenous intron containing its own promoter.Molecular and cellular biology · 2001Article
- Expression of the Naegleria intron endonuclease is dependent on a functional group I self-cleaving ribozyme.RNA (New York, N.Y.) · 2000Article
- Functional alpha-fragment of beta-galactosidase can be expressed from the mobile group I intron PpLSU3 embedded in yeast pre-ribosomal RNA derived from the chromosomal rDNA locus.Nucleic acids research · 2000Article
- In vivo expression of the nucleolar group I intron-encoded I-dirI homing endonuclease involves the removal of a spliceosomal intron.The EMBO journal · 1999Article
- Sequence specificity of in vivo reverse splicing of the Tetrahymena group I intron.RNA (New York, N.Y.) · 1999Article
- I-PpoI, the endonuclease encoded by the group I intron PpLSU3, is expressed from an RNA polymerase I transcript.Molecular and cellular biology · 1998Article
- Gene conversion plays the major role in controlling the stability of large tandem repeats in yeast.The EMBO journal · 1996Article
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2 authors at 1 institution in 1 country.
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Abstract
Pp LSU3 is a mobile group I intron in the extrachromosomal nuclear ribosomal DNA (rDNA) of Physarum polycephalum. As found for other mobile introns, Pp LSU3 encodes a site-specific endonuclease, I-Ppo, which mediates "homing" to unoccupied target sites in Physarum rDNA. The recognition sequence for this enzyme is conserved in all eucaryotic nuclear rDNAs. We have introduced this intron into a heterologous species, Saccharomyces cerevisiae, in which nuclear group I introns have not been detected. The expression of Pp LSU3, under control of the inducible GAL10 promoter, was found to be lethal as a consequence of double-strand breaks in the rDNA. However, surviving colonies that are resistant to the lethal effects of I-Ppo because of alterations in the rDNA at the cleavage site were recovered readily. These survivors are of two classes. The first comprises cells that acquired one of three types of point mutations. The second comprises cells in which Pp LSU3 became inserted into the rDNA. In both cases, each resistant survivor appears to carry the same alterations in all approximately 150 rDNA repeats. When it is embedded in yeast rDNA, Pp LSU3 leads to the synthesis of I-Ppo and appears to be mobile in appropriate genetic crosses. The existence of yeast cells carrying a mobile intron should allow dissection of the steps that allow expression of the highly unusual I-Ppo gene.
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